Flow-cytometric analysis of reactive oxygen species in cancer cells under treatment with brassinosteroids
•Brassinosteroids induced generation of reactive oxygen species (ROS) in A549 cells.•The maximal effect was observed for (22S,23S)-28-homocastasterone.•It exhibited a 6-fold increase of ROS generation at 30μM concentration. To explore the underlying mechanism of cancer cell growth inhibition by bras...
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| Veröffentlicht in: | Steroids 2017-01, Vol.117, p.11-15 |
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| creator | Kisselev, Pyotr A. Panibrat, Olesya V. Sysa, Aliaksei R. Anisovich, Marina V. Zhabinskii, Vladimir N. Khripach, Vladimir A. |
| description | •Brassinosteroids induced generation of reactive oxygen species (ROS) in A549 cells.•The maximal effect was observed for (22S,23S)-28-homocastasterone.•It exhibited a 6-fold increase of ROS generation at 30μM concentration.
To explore the underlying mechanism of cancer cell growth inhibition by brassinosteroids (BS), reactive oxygen species (ROS) generation under treatment with 28-homocastasterone and its synthetic derivatives (22S,23S)-28-homocastasterone was measured in A549 human lung adenocarcinoma cells. BS induced ROS generation in A549 cells and their growth in a time and dose-dependent manner. The maximal effect was observed for (22S,23S)-28-homocastasterone which at 30μM concentration showed a 6-fold increase of ROS generation in comparison with the control. |
| doi_str_mv | 10.1016/j.steroids.2016.06.010 |
| format | Article |
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To explore the underlying mechanism of cancer cell growth inhibition by brassinosteroids (BS), reactive oxygen species (ROS) generation under treatment with 28-homocastasterone and its synthetic derivatives (22S,23S)-28-homocastasterone was measured in A549 human lung adenocarcinoma cells. BS induced ROS generation in A549 cells and their growth in a time and dose-dependent manner. The maximal effect was observed for (22S,23S)-28-homocastasterone which at 30μM concentration showed a 6-fold increase of ROS generation in comparison with the control.</description><identifier>ISSN: 0039-128X</identifier><identifier>EISSN: 1878-5867</identifier><identifier>DOI: 10.1016/j.steroids.2016.06.010</identifier><identifier>PMID: 27343978</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>28-Homocastasterone ; A549 Cells ; Anticancer ; Antineoplastic Agents - pharmacology ; Brassinosteroids ; Brassinosteroids - pharmacology ; Cancer cell line A549 ; Cell Cycle - drug effects ; Cell Line, Tumor ; Cell Survival - drug effects ; Flow Cytometry ; Humans ; Reactive oxygen species ; Reactive Oxygen Species - metabolism</subject><ispartof>Steroids, 2017-01, Vol.117, p.11-15</ispartof><rights>2016 Elsevier Inc.</rights><rights>Copyright © 2016 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-53becabadba3f7800cc74957cb6064468c5fb7e50ed795709fd5614f8ae1cd553</citedby><cites>FETCH-LOGICAL-c368t-53becabadba3f7800cc74957cb6064468c5fb7e50ed795709fd5614f8ae1cd553</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0039128X16300769$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27343978$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kisselev, Pyotr A.</creatorcontrib><creatorcontrib>Panibrat, Olesya V.</creatorcontrib><creatorcontrib>Sysa, Aliaksei R.</creatorcontrib><creatorcontrib>Anisovich, Marina V.</creatorcontrib><creatorcontrib>Zhabinskii, Vladimir N.</creatorcontrib><creatorcontrib>Khripach, Vladimir A.</creatorcontrib><title>Flow-cytometric analysis of reactive oxygen species in cancer cells under treatment with brassinosteroids</title><title>Steroids</title><addtitle>Steroids</addtitle><description>•Brassinosteroids induced generation of reactive oxygen species (ROS) in A549 cells.•The maximal effect was observed for (22S,23S)-28-homocastasterone.•It exhibited a 6-fold increase of ROS generation at 30μM concentration.
To explore the underlying mechanism of cancer cell growth inhibition by brassinosteroids (BS), reactive oxygen species (ROS) generation under treatment with 28-homocastasterone and its synthetic derivatives (22S,23S)-28-homocastasterone was measured in A549 human lung adenocarcinoma cells. BS induced ROS generation in A549 cells and their growth in a time and dose-dependent manner. The maximal effect was observed for (22S,23S)-28-homocastasterone which at 30μM concentration showed a 6-fold increase of ROS generation in comparison with the control.</description><subject>28-Homocastasterone</subject><subject>A549 Cells</subject><subject>Anticancer</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Brassinosteroids</subject><subject>Brassinosteroids - pharmacology</subject><subject>Cancer cell line A549</subject><subject>Cell Cycle - drug effects</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival - drug effects</subject><subject>Flow Cytometry</subject><subject>Humans</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><issn>0039-128X</issn><issn>1878-5867</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1vEzEQhi0EoqHwFyofe9kw3g_bewNVBJAqcQGJm-W1Z6mj3XXqcdrm39dRml6RRvKHnvG8fhi7ErAWIOTn7Zoyphg8retyXkMpAW_YSmilq05L9ZatAJq-ErX-e8E-EG0BQDZ9_Z5d1Kppm17pFQubKT5W7pDjjDkFx-1ipwMF4nHkCa3L4QF5fDr8w4XTDl1A4mHhzi4OE3c4TcT3iy_7XPA845L5Y8h3fEiWKCzxnPMjezfaifDTy3rJ_my-_b75Ud3--v7z5utt5Rqpc9U1Azo7WD_YZlQawDnV9p1ygwTZtlK7bhwUdoBelWvoR99J0Y7aonC-65pLdn16d5fi_R4pmznQMaddMO7JCF1LJYoaWVB5Ql2KRAlHs0thtulgBJijZrM15_jmqNlAKQGl8eplxn6Y0b-2nb0W4MsJwPLTh4DJUDFXlPmQ0GXjY_jfjGftH5Vh</recordid><startdate>201701</startdate><enddate>201701</enddate><creator>Kisselev, Pyotr A.</creator><creator>Panibrat, Olesya V.</creator><creator>Sysa, Aliaksei R.</creator><creator>Anisovich, Marina V.</creator><creator>Zhabinskii, Vladimir N.</creator><creator>Khripach, Vladimir A.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201701</creationdate><title>Flow-cytometric analysis of reactive oxygen species in cancer cells under treatment with brassinosteroids</title><author>Kisselev, Pyotr A. ; Panibrat, Olesya V. ; Sysa, Aliaksei R. ; Anisovich, Marina V. ; Zhabinskii, Vladimir N. ; Khripach, Vladimir A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-53becabadba3f7800cc74957cb6064468c5fb7e50ed795709fd5614f8ae1cd553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>28-Homocastasterone</topic><topic>A549 Cells</topic><topic>Anticancer</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Brassinosteroids</topic><topic>Brassinosteroids - pharmacology</topic><topic>Cancer cell line A549</topic><topic>Cell Cycle - drug effects</topic><topic>Cell Line, Tumor</topic><topic>Cell Survival - drug effects</topic><topic>Flow Cytometry</topic><topic>Humans</topic><topic>Reactive oxygen species</topic><topic>Reactive Oxygen Species - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kisselev, Pyotr A.</creatorcontrib><creatorcontrib>Panibrat, Olesya V.</creatorcontrib><creatorcontrib>Sysa, Aliaksei R.</creatorcontrib><creatorcontrib>Anisovich, Marina V.</creatorcontrib><creatorcontrib>Zhabinskii, Vladimir N.</creatorcontrib><creatorcontrib>Khripach, Vladimir A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Steroids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kisselev, Pyotr A.</au><au>Panibrat, Olesya V.</au><au>Sysa, Aliaksei R.</au><au>Anisovich, Marina V.</au><au>Zhabinskii, Vladimir N.</au><au>Khripach, Vladimir A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Flow-cytometric analysis of reactive oxygen species in cancer cells under treatment with brassinosteroids</atitle><jtitle>Steroids</jtitle><addtitle>Steroids</addtitle><date>2017-01</date><risdate>2017</risdate><volume>117</volume><spage>11</spage><epage>15</epage><pages>11-15</pages><issn>0039-128X</issn><eissn>1878-5867</eissn><abstract>•Brassinosteroids induced generation of reactive oxygen species (ROS) in A549 cells.•The maximal effect was observed for (22S,23S)-28-homocastasterone.•It exhibited a 6-fold increase of ROS generation at 30μM concentration.
To explore the underlying mechanism of cancer cell growth inhibition by brassinosteroids (BS), reactive oxygen species (ROS) generation under treatment with 28-homocastasterone and its synthetic derivatives (22S,23S)-28-homocastasterone was measured in A549 human lung adenocarcinoma cells. BS induced ROS generation in A549 cells and their growth in a time and dose-dependent manner. The maximal effect was observed for (22S,23S)-28-homocastasterone which at 30μM concentration showed a 6-fold increase of ROS generation in comparison with the control.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>27343978</pmid><doi>10.1016/j.steroids.2016.06.010</doi><tpages>5</tpages></addata></record> |
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| subjects | 28-Homocastasterone A549 Cells Anticancer Antineoplastic Agents - pharmacology Brassinosteroids Brassinosteroids - pharmacology Cancer cell line A549 Cell Cycle - drug effects Cell Line, Tumor Cell Survival - drug effects Flow Cytometry Humans Reactive oxygen species Reactive Oxygen Species - metabolism |
| title | Flow-cytometric analysis of reactive oxygen species in cancer cells under treatment with brassinosteroids |
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