Flow-cytometric analysis of reactive oxygen species in cancer cells under treatment with brassinosteroids

•Brassinosteroids induced generation of reactive oxygen species (ROS) in A549 cells.•The maximal effect was observed for (22S,23S)-28-homocastasterone.•It exhibited a 6-fold increase of ROS generation at 30μM concentration. To explore the underlying mechanism of cancer cell growth inhibition by bras...

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Veröffentlicht in:	Steroids 2017-01, Vol.117, p.11-15
Hauptverfasser:	Kisselev, Pyotr A., Panibrat, Olesya V., Sysa, Aliaksei R., Anisovich, Marina V., Zhabinskii, Vladimir N., Khripach, Vladimir A.
Format:	Artikel
Sprache:	eng
Schlagworte:	28-Homocastasterone A549 Cells Anticancer Antineoplastic Agents - pharmacology Brassinosteroids Brassinosteroids - pharmacology Cancer cell line A549 Cell Cycle - drug effects Cell Line, Tumor Cell Survival - drug effects Flow Cytometry Humans Reactive oxygen species Reactive Oxygen Species - metabolism
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Zusammenfassung:	•Brassinosteroids induced generation of reactive oxygen species (ROS) in A549 cells.•The maximal effect was observed for (22S,23S)-28-homocastasterone.•It exhibited a 6-fold increase of ROS generation at 30μM concentration. To explore the underlying mechanism of cancer cell growth inhibition by brassinosteroids (BS), reactive oxygen species (ROS) generation under treatment with 28-homocastasterone and its synthetic derivatives (22S,23S)-28-homocastasterone was measured in A549 human lung adenocarcinoma cells. BS induced ROS generation in A549 cells and their growth in a time and dose-dependent manner. The maximal effect was observed for (22S,23S)-28-homocastasterone which at 30μM concentration showed a 6-fold increase of ROS generation in comparison with the control.
ISSN:	0039-128X 1878-5867
DOI:	10.1016/j.steroids.2016.06.010