Implementation of Hadamard encoding for rapid multisample analysis in liquid chromatography

Multiplexing based on pseudo‐binary modulation sequences is known to increase the signal‐to‐noise ratio. In this work, Hadamard transform multiplexing is used in high‐performance liquid chromatography to increase the sample throughput. Using structured modulation sequences to encode and control sample injections in combination with a fitting algorithm to deconvolute the complex data allowed us to evaluate convoluted chromatograms of up to 128 samples containing three and five analytes, respectively, with good accuracy (