Comparison of two methods for measurement of equine insulin
Diagnosis of equine hyperinsulinemia requires an accurate method for quantification of equine insulin concentrations. The objectives of the current study were to compare 2 commercially available techniques for measurement of equine insulin, the radioimmunoassay (RIA) and chemiluminescent immunoassay...
Gespeichert in:
| Veröffentlicht in: | Journal of veterinary diagnostic investigation 2014-07, Vol.26 (4), p.527-530 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 530 |
|---|---|
| container_issue | 4 |
| container_start_page | 527 |
| container_title | Journal of veterinary diagnostic investigation |
| container_volume | 26 |
| creator | Banse, Heidi E. McCann, Joseph Yang, Fan Wagg, Catherine McFarlane, Dianne |
| description | Diagnosis of equine hyperinsulinemia requires an accurate method for quantification of equine insulin concentrations. The objectives of the current study were to compare 2 commercially available techniques for measurement of equine insulin, the radioimmunoassay (RIA) and chemiluminescent immunoassay (CIA). Recovery was poor for both assays, but worse for the CIA. Serial dilution of a high endogenous insulin sample yielded better linearity for the RIA (r2 = 0.99, P < 0.001) than the CIA (r2 = 0.92, P = 0.009). Bland–Altman analysis indicated that the CIA was, on average, 91 pmol/l higher than the RIA, with wide limits of agreement (95% limits of agreement: –508 to 691 pmol/l). These findings suggest that results between the assays should not be considered interchangeable. |
| doi_str_mv | 10.1177/1040638714536560 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1826594384</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.1177_1040638714536560</sage_id><sourcerecordid>1826594384</sourcerecordid><originalsourceid>FETCH-LOGICAL-c379t-ff9ccbe3e97a53db0c5c6f2090e3f701f61ac3bfbf9fefa2c70b8cb6be5939b93</originalsourceid><addsrcrecordid>eNp1kM1LAzEUxIMotlbvnmSPXlaTzSbZ4ElK_YCCFz2HJH3RLbubNtkg_vemtHoQPL2B-c3AG4QuCb4hRIhbgmvMaSNIzShnHB-hKZE1LWtJ-XHW2S53_gSdxbjGmFVMkFM0qWpZNUw2U3Q39_1Ghzb6ofCuGD990cP44VexcD5krWMK0MMw7mzYpnaAoh1i6trhHJ043UW4ONwZentYvM6fyuXL4_P8fllaKuRYOietNUBBCs3oymDLLHcVlhioE5g4TrSlxhknHThdWYFNYw03wCSVRtIZut73boLfJoij6ttooev0AD5FRZqKs_x2U2cU71EbfIwBnNqEttfhSxGsdpOpv5PlyNWhPZkeVr-Bn40yUO6BqN9BrX0KQ_72_8JvEZh0TQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1826594384</pqid></control><display><type>article</type><title>Comparison of two methods for measurement of equine insulin</title><source>Access via SAGE</source><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Banse, Heidi E. ; McCann, Joseph ; Yang, Fan ; Wagg, Catherine ; McFarlane, Dianne</creator><creatorcontrib>Banse, Heidi E. ; McCann, Joseph ; Yang, Fan ; Wagg, Catherine ; McFarlane, Dianne</creatorcontrib><description>Diagnosis of equine hyperinsulinemia requires an accurate method for quantification of equine insulin concentrations. The objectives of the current study were to compare 2 commercially available techniques for measurement of equine insulin, the radioimmunoassay (RIA) and chemiluminescent immunoassay (CIA). Recovery was poor for both assays, but worse for the CIA. Serial dilution of a high endogenous insulin sample yielded better linearity for the RIA (r2 = 0.99, P < 0.001) than the CIA (r2 = 0.92, P = 0.009). Bland–Altman analysis indicated that the CIA was, on average, 91 pmol/l higher than the RIA, with wide limits of agreement (95% limits of agreement: –508 to 691 pmol/l). These findings suggest that results between the assays should not be considered interchangeable.</description><identifier>ISSN: 1040-6387</identifier><identifier>EISSN: 1943-4936</identifier><identifier>DOI: 10.1177/1040638714536560</identifier><identifier>PMID: 24928598</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Animals ; Horses - metabolism ; Insulin - metabolism ; Luminescent Measurements - methods ; Luminescent Measurements - veterinary ; Radioimmunoassay - methods ; Radioimmunoassay - veterinary ; Reproducibility of Results ; Sensitivity and Specificity</subject><ispartof>Journal of veterinary diagnostic investigation, 2014-07, Vol.26 (4), p.527-530</ispartof><rights>2014 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c379t-ff9ccbe3e97a53db0c5c6f2090e3f701f61ac3bfbf9fefa2c70b8cb6be5939b93</citedby><cites>FETCH-LOGICAL-c379t-ff9ccbe3e97a53db0c5c6f2090e3f701f61ac3bfbf9fefa2c70b8cb6be5939b93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/1040638714536560$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/1040638714536560$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,780,784,21819,27924,27925,43621,43622</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24928598$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Banse, Heidi E.</creatorcontrib><creatorcontrib>McCann, Joseph</creatorcontrib><creatorcontrib>Yang, Fan</creatorcontrib><creatorcontrib>Wagg, Catherine</creatorcontrib><creatorcontrib>McFarlane, Dianne</creatorcontrib><title>Comparison of two methods for measurement of equine insulin</title><title>Journal of veterinary diagnostic investigation</title><addtitle>J Vet Diagn Invest</addtitle><description>Diagnosis of equine hyperinsulinemia requires an accurate method for quantification of equine insulin concentrations. The objectives of the current study were to compare 2 commercially available techniques for measurement of equine insulin, the radioimmunoassay (RIA) and chemiluminescent immunoassay (CIA). Recovery was poor for both assays, but worse for the CIA. Serial dilution of a high endogenous insulin sample yielded better linearity for the RIA (r2 = 0.99, P < 0.001) than the CIA (r2 = 0.92, P = 0.009). Bland–Altman analysis indicated that the CIA was, on average, 91 pmol/l higher than the RIA, with wide limits of agreement (95% limits of agreement: –508 to 691 pmol/l). These findings suggest that results between the assays should not be considered interchangeable.</description><subject>Animals</subject><subject>Horses - metabolism</subject><subject>Insulin - metabolism</subject><subject>Luminescent Measurements - methods</subject><subject>Luminescent Measurements - veterinary</subject><subject>Radioimmunoassay - methods</subject><subject>Radioimmunoassay - veterinary</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><issn>1040-6387</issn><issn>1943-4936</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1LAzEUxIMotlbvnmSPXlaTzSbZ4ElK_YCCFz2HJH3RLbubNtkg_vemtHoQPL2B-c3AG4QuCb4hRIhbgmvMaSNIzShnHB-hKZE1LWtJ-XHW2S53_gSdxbjGmFVMkFM0qWpZNUw2U3Q39_1Ghzb6ofCuGD990cP44VexcD5krWMK0MMw7mzYpnaAoh1i6trhHJ043UW4ONwZentYvM6fyuXL4_P8fllaKuRYOietNUBBCs3oymDLLHcVlhioE5g4TrSlxhknHThdWYFNYw03wCSVRtIZut73boLfJoij6ttooev0AD5FRZqKs_x2U2cU71EbfIwBnNqEttfhSxGsdpOpv5PlyNWhPZkeVr-Bn40yUO6BqN9BrX0KQ_72_8JvEZh0TQ</recordid><startdate>20140701</startdate><enddate>20140701</enddate><creator>Banse, Heidi E.</creator><creator>McCann, Joseph</creator><creator>Yang, Fan</creator><creator>Wagg, Catherine</creator><creator>McFarlane, Dianne</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140701</creationdate><title>Comparison of two methods for measurement of equine insulin</title><author>Banse, Heidi E. ; McCann, Joseph ; Yang, Fan ; Wagg, Catherine ; McFarlane, Dianne</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c379t-ff9ccbe3e97a53db0c5c6f2090e3f701f61ac3bfbf9fefa2c70b8cb6be5939b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Horses - metabolism</topic><topic>Insulin - metabolism</topic><topic>Luminescent Measurements - methods</topic><topic>Luminescent Measurements - veterinary</topic><topic>Radioimmunoassay - methods</topic><topic>Radioimmunoassay - veterinary</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Banse, Heidi E.</creatorcontrib><creatorcontrib>McCann, Joseph</creatorcontrib><creatorcontrib>Yang, Fan</creatorcontrib><creatorcontrib>Wagg, Catherine</creatorcontrib><creatorcontrib>McFarlane, Dianne</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of veterinary diagnostic investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Banse, Heidi E.</au><au>McCann, Joseph</au><au>Yang, Fan</au><au>Wagg, Catherine</au><au>McFarlane, Dianne</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of two methods for measurement of equine insulin</atitle><jtitle>Journal of veterinary diagnostic investigation</jtitle><addtitle>J Vet Diagn Invest</addtitle><date>2014-07-01</date><risdate>2014</risdate><volume>26</volume><issue>4</issue><spage>527</spage><epage>530</epage><pages>527-530</pages><issn>1040-6387</issn><eissn>1943-4936</eissn><abstract>Diagnosis of equine hyperinsulinemia requires an accurate method for quantification of equine insulin concentrations. The objectives of the current study were to compare 2 commercially available techniques for measurement of equine insulin, the radioimmunoassay (RIA) and chemiluminescent immunoassay (CIA). Recovery was poor for both assays, but worse for the CIA. Serial dilution of a high endogenous insulin sample yielded better linearity for the RIA (r2 = 0.99, P < 0.001) than the CIA (r2 = 0.92, P = 0.009). Bland–Altman analysis indicated that the CIA was, on average, 91 pmol/l higher than the RIA, with wide limits of agreement (95% limits of agreement: –508 to 691 pmol/l). These findings suggest that results between the assays should not be considered interchangeable.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>24928598</pmid><doi>10.1177/1040638714536560</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1040-6387 |
| ispartof | Journal of veterinary diagnostic investigation, 2014-07, Vol.26 (4), p.527-530 |
| issn | 1040-6387 1943-4936 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1826594384 |
| source | Access via SAGE; MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Horses - metabolism Insulin - metabolism Luminescent Measurements - methods Luminescent Measurements - veterinary Radioimmunoassay - methods Radioimmunoassay - veterinary Reproducibility of Results Sensitivity and Specificity |
| title | Comparison of two methods for measurement of equine insulin |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T11%3A54%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparison%20of%20two%20methods%20for%20measurement%20of%20equine%20insulin&rft.jtitle=Journal%20of%20veterinary%20diagnostic%20investigation&rft.au=Banse,%20Heidi%20E.&rft.date=2014-07-01&rft.volume=26&rft.issue=4&rft.spage=527&rft.epage=530&rft.pages=527-530&rft.issn=1040-6387&rft.eissn=1943-4936&rft_id=info:doi/10.1177/1040638714536560&rft_dat=%3Cproquest_cross%3E1826594384%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1826594384&rft_id=info:pmid/24928598&rft_sage_id=10.1177_1040638714536560&rfr_iscdi=true |