Effect of growth regulator and culture conditions on shoot multiplication and rhizome formation in ginger (Zingiber officinale Rosc.) in vitro
Shoot multiplication of Zingiber officinale cv. V sub(3)S sub(18) was achieved by meristem culture on a Murashige and Skoog (MS) basal medium supplemented with 26.6 mu M 6-benzylaminopurine (BA), 8.57 mu M indole-3-acetic acid (IAA), and 1111.1 mu M adenine sulfate and 3% (w/v) sucrose. In vitro rhi...
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Veröffentlicht in: | In vitro cellular & developmental biology. Plant 2001-12, Vol.37 (6), p.814-819 |
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Sprache: | eng |
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Zusammenfassung: | Shoot multiplication of Zingiber officinale cv. V sub(3)S sub(18) was achieved by meristem culture on a Murashige and Skoog (MS) basal medium supplemented with 26.6 mu M 6-benzylaminopurine (BA), 8.57 mu M indole-3-acetic acid (IAA), and 1111.1 mu M adenine sulfate and 3% (w/v) sucrose. In vitro rhizome formation from in vitro-raised shoots was achieved on MS medium supplemented with 4.44 mu M BA, 5.71 mu M IAA, and 3-8% (w/v) sucrose after 8 wk of culture. Cultural variations such as photoperiod, carbohydrate, nutrient composition, and growth regulators were tested for the maximum yield of rhizomes. Among the different photoperiods used, a 24-h photoperiod helped in the formation of more rhizomes as compared with other photoperiods. Of the different carbohydrates used, sucrose helped to achieve rhizome formation as compared to other carbohydrates. The microrhizomes sprouted in a soil mixture within 2 wk of planting. The sprouted plantlets survived under field conditions with normal growth. |
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ISSN: | 1054-5476 |
DOI: | 10.1079/IVP2001217 |