Development of a Biosensor Based on Phosphotriesterase and n-Channel ISFET for Detection of Pesticides

A biosensor for the direct determination of organophosphorus pesticides (OPs) is described. It utilizes an ion‐selective field‐effect transistor and phosphotriesterase enzyme (PTE), which provides cleavage of OPs. The sensitivity of the biosensor was increased after PTE immobilization on the surface...

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Veröffentlicht in:Electroanalysis (New York, N.Y.) N.Y.), 2016-06, Vol.28 (6), p.1311-1321
Hauptverfasser: Andrianova, M. S., Gubanova, O. V., Komarova, N. V., Kuznetsov, E. V., Kuznetsov, A. E.
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Sprache:eng
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Zusammenfassung:A biosensor for the direct determination of organophosphorus pesticides (OPs) is described. It utilizes an ion‐selective field‐effect transistor and phosphotriesterase enzyme (PTE), which provides cleavage of OPs. The sensitivity of the biosensor was increased after PTE immobilization on the surface of the transistor. Packaging of the integrated circuit and creation of a microfluidic system for analyte delivery stabilized the signal. This system was able to detect 0.1 µM paraoxon and 0.5 µM parathion and methyl parathion and was stable for at least 1 month (PBS, 1°), and could thus provide the basis for a portable device for analyzing OPs in water.
ISSN:1040-0397
1521-4109
DOI:10.1002/elan.201500411