Cytotoxic effects of hyperthermia, chemotherapy (Navelbine) and radiation on glioma spheroids

Glioblastoma multiform (GBM) is the most common malignant primary brain tumor in humans. Current conventional treatment could not effectively increase the survival rate of patients who suffered from GBM. In clinical view, some experiments which can mimic in vivo conditions to evaluate anticancer agent effects on tumors are very useful. We can fairly reach to this goal using 3-dimentional spheroids rather than monolayer culture. The aim of this study was to determine the cytotoxicity caused by Navelbine, hyperthermia and radiation on glioma spheroids. The spheroid cells were separately or simultaneously treated with the doses of 7.5μg/ml Navelbine for 50.3h, irradiated with 2Gy and incubated at 43°C for 1h. Comet and colony formation assays were applied to assess the DNA damages and survival rate of the cells. In both comet and colony formation assays, cell damage in all treated groups was significantly higher in comparison with control group (P