Fecal Microbiota in Patients with Irritable Bowel Syndrome Compared with Healthy Controls Using Real-Time Polymerase Chain Reaction: An Evidence of Dysbiosis
Background Dysbiosis may play a role in irritable bowel syndrome (IBS), hitherto an enigmatic disorder. We evaluated selected fecal microbes in IBS patients and healthy controls (HC). Methods Fecal 16S rRNA copy number of selected bacteria was studied using qPCR in 47 patients with IBS (Rome III) an...
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| description | Background
Dysbiosis may play a role in irritable bowel syndrome (IBS), hitherto an enigmatic disorder. We evaluated selected fecal microbes in IBS patients and healthy controls (HC).
Methods
Fecal 16S rRNA copy number of selected bacteria was studied using qPCR in 47 patients with IBS (Rome III) and 30 HC.
Results
Of 47 patients, 20 had constipation (IBS-C), 20 diarrhea (IBS-D), and seven unclassified IBS (IBS-U). Relative difference in 16S rRNA copy number of
Bifidobacterium
(
P
= 0.042) was lower, while those of
Ruminococcus productus
-
Clostridium coccoides
(
P
= 0.016),
Veillonella
(
P
= 0.008),
Bacteroides thetaiotamicron
(
P
|
| doi_str_mv | 10.1007/s10620-015-3607-y |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_1823943105</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A712943754</galeid><sourcerecordid>A712943754</sourcerecordid><originalsourceid>FETCH-LOGICAL-c542t-de68d335abc467084966bbde143026ebb68cbf9b19a516bf5a1c060c15a42d8d3</originalsourceid><addsrcrecordid>eNqFkstu1DAUhiNERYfCA7BBltiwSWs7sZ2wG4aWViqignZt2c7JjKvEntoeqjwM74qjKVeBkBe2zvn-c5H_onhB8DHBWJxEgjnFJSasrDgW5fSoWBAmqpIy3jwuFpjw_CaEHxZPY7zFGLeC8CfFIWWiqbGoF8XXMzBqQB-sCV5bnxSyDl2pZMGliO5t2qCLEGxSegD01t_DgD5Prgt-BLTy41YF6PbYOaghbaYcdSn4IaKbaN0afcrh8tpm_MoP0whBxazcqNwmp0yy3r1BS4dOv9gOnAHke_RuinmWaOOz4qBXQ4TnD_dRcXN2er06Ly8_vr9YLS9Lw2qayg5401UVU9rUXOCmbjnXugNSV5hy0Jo3RvetJq1ihOueKWIwx4YwVdMuS4-K1_u62-DvdhCTHG00MAzKgd9FSRpatXVFMPs_KkjVsorSOqOv_kBv_S64vMhMUUEbTKuf1FoNIK3rfQrKzEXlMlO5rWBzreO_UPl0MFrjHfQ2x38TkL0gf2yMAXq5DXZUYZIEy9k9cu8emd0jZ_fIKWtePgy80yN0PxTf7ZIBugdiTrk1hF82-mfVb68LzyE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1712728023</pqid></control><display><type>article</type><title>Fecal Microbiota in Patients with Irritable Bowel Syndrome Compared with Healthy Controls Using Real-Time Polymerase Chain Reaction: An Evidence of Dysbiosis</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Shukla, Ratnakar ; Ghoshal, Ujjala ; Dhole, Tapan N. ; Ghoshal, Uday C.</creator><creatorcontrib>Shukla, Ratnakar ; Ghoshal, Ujjala ; Dhole, Tapan N. ; Ghoshal, Uday C.</creatorcontrib><description><![CDATA[Background
Dysbiosis may play a role in irritable bowel syndrome (IBS), hitherto an enigmatic disorder. We evaluated selected fecal microbes in IBS patients and healthy controls (HC).
Methods
Fecal 16S rRNA copy number of selected bacteria was studied using qPCR in 47 patients with IBS (Rome III) and 30 HC.
Results
Of 47 patients, 20 had constipation (IBS-C), 20 diarrhea (IBS-D), and seven unclassified IBS (IBS-U). Relative difference in 16S rRNA copy number of
Bifidobacterium
(
P
= 0.042) was lower, while those of
Ruminococcus productus
-
Clostridium coccoides
(
P
= 0.016),
Veillonella
(
P
= 0.008),
Bacteroides thetaiotamicron
(
P
< 0.001),
Pseudomonas aeruginosa
(
P
< 0.001), and Gram-negative bacteria (GNB,
P
= 0.001) were higher among IBS patients than HC. Number of
Lactobacillus
(
P
= 0.002) was lower, while that of
Bacteroides thetaiotamicron
(
P
< 0.001) and segmented filamentous bacteria (SFB,
P
< 0.001) was higher among IBS-D than IBS-C. Numbers of
Bacteroides thetaiotamicron
(
P
< 0.001),
P. aeruginosa
(
P
< 0.001), and GNB (
P
< 0.01) were higher among IBS-C and IBS-D than HC. Quantity of SFB was higher among IBS-D (
P
= 0.011) and lower among IBS-C (
P
= 0.002) than HC. Number of
Veillonella
species was higher among IBS-C than HC (
P
= 0.002).
P. aeruginosa
was frequently detected among IBS than HC (46/47 [97.9 %] vs. 10/30 [33.3 %],
P
< 0.001). Abdominal distension (
n
= 34/47) was associated with higher number of
Bacteroides thetaiotamicron
,
Clostridium coccoides
,
P. aeruginosa
, SFB, and GNB; bloating (
n
= 22/47) was associated with
Clostridium coccoides
and GNB. Microbial flora was different among IBS than HC on principal component analysis.
Conclusion
Fecal microbiota was different among IBS than HC, and different sub-types were associated with different microbiota.
P. aeruginosa
was more frequent and higher in number among IBS patients.]]></description><identifier>ISSN: 0163-2116</identifier><identifier>EISSN: 1573-2568</identifier><identifier>DOI: 10.1007/s10620-015-3607-y</identifier><identifier>PMID: 25784074</identifier><identifier>CODEN: DDSCDJ</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Adult ; Aged ; Bacteria ; Bacteroides thetaiotamicron ; Bifidobacterium ; Biochemistry ; Care and treatment ; Case-Control Studies ; Clostridium coccoides ; Comparative analysis ; Diarrhea ; DNA, Bacterial - analysis ; Dysbiosis - microbiology ; Evidence-Based Medicine ; Feces - microbiology ; Female ; Gastroenterology ; Gram-Negative Bacteria - genetics ; Gram-Negative Bacteria - isolation & purification ; Gram-Positive Bacteria - genetics ; Gram-Positive Bacteria - isolation & purification ; Hepatology ; Humans ; Irritable bowel syndrome ; Irritable Bowel Syndrome - microbiology ; Irritable Bowel Syndrome - physiopathology ; Lactobacillus ; Male ; Medical research ; Medicine ; Medicine & Public Health ; Medicine, Experimental ; Microbiota (Symbiotic organisms) ; Microbiota - genetics ; Middle Aged ; Oncology ; Original Article ; Polymerase chain reaction ; Pseudomonas aeruginosa ; Real-Time Polymerase Chain Reaction - methods ; Reference Values ; RNA ; Ruminococcus ; Transplant Surgery ; Veillonella ; Young Adult</subject><ispartof>Digestive diseases and sciences, 2015-10, Vol.60 (10), p.2953-2962</ispartof><rights>Springer Science+Business Media New York 2015</rights><rights>COPYRIGHT 2015 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c542t-de68d335abc467084966bbde143026ebb68cbf9b19a516bf5a1c060c15a42d8d3</citedby><cites>FETCH-LOGICAL-c542t-de68d335abc467084966bbde143026ebb68cbf9b19a516bf5a1c060c15a42d8d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10620-015-3607-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10620-015-3607-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25784074$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shukla, Ratnakar</creatorcontrib><creatorcontrib>Ghoshal, Ujjala</creatorcontrib><creatorcontrib>Dhole, Tapan N.</creatorcontrib><creatorcontrib>Ghoshal, Uday C.</creatorcontrib><title>Fecal Microbiota in Patients with Irritable Bowel Syndrome Compared with Healthy Controls Using Real-Time Polymerase Chain Reaction: An Evidence of Dysbiosis</title><title>Digestive diseases and sciences</title><addtitle>Dig Dis Sci</addtitle><addtitle>Dig Dis Sci</addtitle><description><![CDATA[Background
Dysbiosis may play a role in irritable bowel syndrome (IBS), hitherto an enigmatic disorder. We evaluated selected fecal microbes in IBS patients and healthy controls (HC).
Methods
Fecal 16S rRNA copy number of selected bacteria was studied using qPCR in 47 patients with IBS (Rome III) and 30 HC.
Results
Of 47 patients, 20 had constipation (IBS-C), 20 diarrhea (IBS-D), and seven unclassified IBS (IBS-U). Relative difference in 16S rRNA copy number of
Bifidobacterium
(
P
= 0.042) was lower, while those of
Ruminococcus productus
-
Clostridium coccoides
(
P
= 0.016),
Veillonella
(
P
= 0.008),
Bacteroides thetaiotamicron
(
P
< 0.001),
Pseudomonas aeruginosa
(
P
< 0.001), and Gram-negative bacteria (GNB,
P
= 0.001) were higher among IBS patients than HC. Number of
Lactobacillus
(
P
= 0.002) was lower, while that of
Bacteroides thetaiotamicron
(
P
< 0.001) and segmented filamentous bacteria (SFB,
P
< 0.001) was higher among IBS-D than IBS-C. Numbers of
Bacteroides thetaiotamicron
(
P
< 0.001),
P. aeruginosa
(
P
< 0.001), and GNB (
P
< 0.01) were higher among IBS-C and IBS-D than HC. Quantity of SFB was higher among IBS-D (
P
= 0.011) and lower among IBS-C (
P
= 0.002) than HC. Number of
Veillonella
species was higher among IBS-C than HC (
P
= 0.002).
P. aeruginosa
was frequently detected among IBS than HC (46/47 [97.9 %] vs. 10/30 [33.3 %],
P
< 0.001). Abdominal distension (
n
= 34/47) was associated with higher number of
Bacteroides thetaiotamicron
,
Clostridium coccoides
,
P. aeruginosa
, SFB, and GNB; bloating (
n
= 22/47) was associated with
Clostridium coccoides
and GNB. Microbial flora was different among IBS than HC on principal component analysis.
Conclusion
Fecal microbiota was different among IBS than HC, and different sub-types were associated with different microbiota.
P. aeruginosa
was more frequent and higher in number among IBS patients.]]></description><subject>Adult</subject><subject>Aged</subject><subject>Bacteria</subject><subject>Bacteroides thetaiotamicron</subject><subject>Bifidobacterium</subject><subject>Biochemistry</subject><subject>Care and treatment</subject><subject>Case-Control Studies</subject><subject>Clostridium coccoides</subject><subject>Comparative analysis</subject><subject>Diarrhea</subject><subject>DNA, Bacterial - analysis</subject><subject>Dysbiosis - microbiology</subject><subject>Evidence-Based Medicine</subject><subject>Feces - microbiology</subject><subject>Female</subject><subject>Gastroenterology</subject><subject>Gram-Negative Bacteria - genetics</subject><subject>Gram-Negative Bacteria - isolation & purification</subject><subject>Gram-Positive Bacteria - genetics</subject><subject>Gram-Positive Bacteria - isolation & purification</subject><subject>Hepatology</subject><subject>Humans</subject><subject>Irritable bowel syndrome</subject><subject>Irritable Bowel Syndrome - microbiology</subject><subject>Irritable Bowel Syndrome - physiopathology</subject><subject>Lactobacillus</subject><subject>Male</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Medicine, Experimental</subject><subject>Microbiota (Symbiotic organisms)</subject><subject>Microbiota - genetics</subject><subject>Middle Aged</subject><subject>Oncology</subject><subject>Original Article</subject><subject>Polymerase chain reaction</subject><subject>Pseudomonas aeruginosa</subject><subject>Real-Time Polymerase Chain Reaction - methods</subject><subject>Reference Values</subject><subject>RNA</subject><subject>Ruminococcus</subject><subject>Transplant Surgery</subject><subject>Veillonella</subject><subject>Young Adult</subject><issn>0163-2116</issn><issn>1573-2568</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkstu1DAUhiNERYfCA7BBltiwSWs7sZ2wG4aWViqignZt2c7JjKvEntoeqjwM74qjKVeBkBe2zvn-c5H_onhB8DHBWJxEgjnFJSasrDgW5fSoWBAmqpIy3jwuFpjw_CaEHxZPY7zFGLeC8CfFIWWiqbGoF8XXMzBqQB-sCV5bnxSyDl2pZMGliO5t2qCLEGxSegD01t_DgD5Prgt-BLTy41YF6PbYOaghbaYcdSn4IaKbaN0afcrh8tpm_MoP0whBxazcqNwmp0yy3r1BS4dOv9gOnAHke_RuinmWaOOz4qBXQ4TnD_dRcXN2er06Ly8_vr9YLS9Lw2qayg5401UVU9rUXOCmbjnXugNSV5hy0Jo3RvetJq1ihOueKWIwx4YwVdMuS4-K1_u62-DvdhCTHG00MAzKgd9FSRpatXVFMPs_KkjVsorSOqOv_kBv_S64vMhMUUEbTKuf1FoNIK3rfQrKzEXlMlO5rWBzreO_UPl0MFrjHfQ2x38TkL0gf2yMAXq5DXZUYZIEy9k9cu8emd0jZ_fIKWtePgy80yN0PxTf7ZIBugdiTrk1hF82-mfVb68LzyE</recordid><startdate>20151001</startdate><enddate>20151001</enddate><creator>Shukla, Ratnakar</creator><creator>Ghoshal, Ujjala</creator><creator>Dhole, Tapan N.</creator><creator>Ghoshal, Uday C.</creator><general>Springer US</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20151001</creationdate><title>Fecal Microbiota in Patients with Irritable Bowel Syndrome Compared with Healthy Controls Using Real-Time Polymerase Chain Reaction: An Evidence of Dysbiosis</title><author>Shukla, Ratnakar ; Ghoshal, Ujjala ; Dhole, Tapan N. ; Ghoshal, Uday C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c542t-de68d335abc467084966bbde143026ebb68cbf9b19a516bf5a1c060c15a42d8d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Bacteria</topic><topic>Bacteroides thetaiotamicron</topic><topic>Bifidobacterium</topic><topic>Biochemistry</topic><topic>Care and treatment</topic><topic>Case-Control Studies</topic><topic>Clostridium coccoides</topic><topic>Comparative analysis</topic><topic>Diarrhea</topic><topic>DNA, Bacterial - analysis</topic><topic>Dysbiosis - microbiology</topic><topic>Evidence-Based Medicine</topic><topic>Feces - microbiology</topic><topic>Female</topic><topic>Gastroenterology</topic><topic>Gram-Negative Bacteria - genetics</topic><topic>Gram-Negative Bacteria - isolation & purification</topic><topic>Gram-Positive Bacteria - genetics</topic><topic>Gram-Positive Bacteria - isolation & purification</topic><topic>Hepatology</topic><topic>Humans</topic><topic>Irritable bowel syndrome</topic><topic>Irritable Bowel Syndrome - microbiology</topic><topic>Irritable Bowel Syndrome - physiopathology</topic><topic>Lactobacillus</topic><topic>Male</topic><topic>Medical research</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Medicine, Experimental</topic><topic>Microbiota (Symbiotic organisms)</topic><topic>Microbiota - genetics</topic><topic>Middle Aged</topic><topic>Oncology</topic><topic>Original Article</topic><topic>Polymerase chain reaction</topic><topic>Pseudomonas aeruginosa</topic><topic>Real-Time Polymerase Chain Reaction - methods</topic><topic>Reference Values</topic><topic>RNA</topic><topic>Ruminococcus</topic><topic>Transplant Surgery</topic><topic>Veillonella</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shukla, Ratnakar</creatorcontrib><creatorcontrib>Ghoshal, Ujjala</creatorcontrib><creatorcontrib>Dhole, Tapan N.</creatorcontrib><creatorcontrib>Ghoshal, Uday C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Digestive diseases and sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shukla, Ratnakar</au><au>Ghoshal, Ujjala</au><au>Dhole, Tapan N.</au><au>Ghoshal, Uday C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fecal Microbiota in Patients with Irritable Bowel Syndrome Compared with Healthy Controls Using Real-Time Polymerase Chain Reaction: An Evidence of Dysbiosis</atitle><jtitle>Digestive diseases and sciences</jtitle><stitle>Dig Dis Sci</stitle><addtitle>Dig Dis Sci</addtitle><date>2015-10-01</date><risdate>2015</risdate><volume>60</volume><issue>10</issue><spage>2953</spage><epage>2962</epage><pages>2953-2962</pages><issn>0163-2116</issn><eissn>1573-2568</eissn><coden>DDSCDJ</coden><abstract><![CDATA[Background
Dysbiosis may play a role in irritable bowel syndrome (IBS), hitherto an enigmatic disorder. We evaluated selected fecal microbes in IBS patients and healthy controls (HC).
Methods
Fecal 16S rRNA copy number of selected bacteria was studied using qPCR in 47 patients with IBS (Rome III) and 30 HC.
Results
Of 47 patients, 20 had constipation (IBS-C), 20 diarrhea (IBS-D), and seven unclassified IBS (IBS-U). Relative difference in 16S rRNA copy number of
Bifidobacterium
(
P
= 0.042) was lower, while those of
Ruminococcus productus
-
Clostridium coccoides
(
P
= 0.016),
Veillonella
(
P
= 0.008),
Bacteroides thetaiotamicron
(
P
< 0.001),
Pseudomonas aeruginosa
(
P
< 0.001), and Gram-negative bacteria (GNB,
P
= 0.001) were higher among IBS patients than HC. Number of
Lactobacillus
(
P
= 0.002) was lower, while that of
Bacteroides thetaiotamicron
(
P
< 0.001) and segmented filamentous bacteria (SFB,
P
< 0.001) was higher among IBS-D than IBS-C. Numbers of
Bacteroides thetaiotamicron
(
P
< 0.001),
P. aeruginosa
(
P
< 0.001), and GNB (
P
< 0.01) were higher among IBS-C and IBS-D than HC. Quantity of SFB was higher among IBS-D (
P
= 0.011) and lower among IBS-C (
P
= 0.002) than HC. Number of
Veillonella
species was higher among IBS-C than HC (
P
= 0.002).
P. aeruginosa
was frequently detected among IBS than HC (46/47 [97.9 %] vs. 10/30 [33.3 %],
P
< 0.001). Abdominal distension (
n
= 34/47) was associated with higher number of
Bacteroides thetaiotamicron
,
Clostridium coccoides
,
P. aeruginosa
, SFB, and GNB; bloating (
n
= 22/47) was associated with
Clostridium coccoides
and GNB. Microbial flora was different among IBS than HC on principal component analysis.
Conclusion
Fecal microbiota was different among IBS than HC, and different sub-types were associated with different microbiota.
P. aeruginosa
was more frequent and higher in number among IBS patients.]]></abstract><cop>New York</cop><pub>Springer US</pub><pmid>25784074</pmid><doi>10.1007/s10620-015-3607-y</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Digestive diseases and sciences, 2015-10, Vol.60 (10), p.2953-2962 |
| issn | 0163-2116 1573-2568 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Adult Aged Bacteria Bacteroides thetaiotamicron Bifidobacterium Biochemistry Care and treatment Case-Control Studies Clostridium coccoides Comparative analysis Diarrhea DNA, Bacterial - analysis Dysbiosis - microbiology Evidence-Based Medicine Feces - microbiology Female Gastroenterology Gram-Negative Bacteria - genetics Gram-Negative Bacteria - isolation & purification Gram-Positive Bacteria - genetics Gram-Positive Bacteria - isolation & purification Hepatology Humans Irritable bowel syndrome Irritable Bowel Syndrome - microbiology Irritable Bowel Syndrome - physiopathology Lactobacillus Male Medical research Medicine Medicine & Public Health Medicine, Experimental Microbiota (Symbiotic organisms) Microbiota - genetics Middle Aged Oncology Original Article Polymerase chain reaction Pseudomonas aeruginosa Real-Time Polymerase Chain Reaction - methods Reference Values RNA Ruminococcus Transplant Surgery Veillonella Young Adult |
| title | Fecal Microbiota in Patients with Irritable Bowel Syndrome Compared with Healthy Controls Using Real-Time Polymerase Chain Reaction: An Evidence of Dysbiosis |
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