Fecal Microbiota in Patients with Irritable Bowel Syndrome Compared with Healthy Controls Using Real-Time Polymerase Chain Reaction: An Evidence of Dysbiosis

Background Dysbiosis may play a role in irritable bowel syndrome (IBS), hitherto an enigmatic disorder. We evaluated selected fecal microbes in IBS patients and healthy controls (HC). Methods Fecal 16S rRNA copy number of selected bacteria was studied using qPCR in 47 patients with IBS (Rome III) an...

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Veröffentlicht in:	Digestive diseases and sciences 2015-10, Vol.60 (10), p.2953-2962
Hauptverfasser:	Shukla, Ratnakar, Ghoshal, Ujjala, Dhole, Tapan N., Ghoshal, Uday C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Aged Bacteria Bacteroides thetaiotamicron Bifidobacterium Biochemistry Care and treatment Case-Control Studies Clostridium coccoides Comparative analysis Diarrhea DNA, Bacterial - analysis Dysbiosis - microbiology Evidence-Based Medicine Feces - microbiology Female Gastroenterology Gram-Negative Bacteria - genetics Gram-Negative Bacteria - isolation & purification Gram-Positive Bacteria - genetics Gram-Positive Bacteria - isolation & purification Hepatology Humans Irritable bowel syndrome Irritable Bowel Syndrome - microbiology Irritable Bowel Syndrome - physiopathology Lactobacillus Male Medical research Medicine Medicine & Public Health Medicine, Experimental Microbiota (Symbiotic organisms) Microbiota - genetics Middle Aged Oncology Original Article Polymerase chain reaction Pseudomonas aeruginosa Real-Time Polymerase Chain Reaction - methods Reference Values RNA Ruminococcus Transplant Surgery Veillonella Young Adult
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Zusammenfassung:	Background Dysbiosis may play a role in irritable bowel syndrome (IBS), hitherto an enigmatic disorder. We evaluated selected fecal microbes in IBS patients and healthy controls (HC). Methods Fecal 16S rRNA copy number of selected bacteria was studied using qPCR in 47 patients with IBS (Rome III) and 30 HC. Results Of 47 patients, 20 had constipation (IBS-C), 20 diarrhea (IBS-D), and seven unclassified IBS (IBS-U). Relative difference in 16S rRNA copy number of Bifidobacterium ( P = 0.042) was lower, while those of Ruminococcus productus - Clostridium coccoides ( P = 0.016), Veillonella ( P = 0.008), Bacteroides thetaiotamicron ( P
ISSN:	0163-2116 1573-2568
DOI:	10.1007/s10620-015-3607-y