Characterization of hepcidin response to holotransferrin in novel recombinant TfR1 HepG2 cells

Hepcidin is the key regulator of systemic iron homeostasis. The iron-sensing mechanisms and the role of intracellular iron in modulating hepatic hepcidin secretion are unclear. Therefore, we created a novel cell line, recombinant-TfR1 HepG2, expressing iron-response-element-independent TFRC mRNA to...

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Veröffentlicht in:	Blood cells, molecules, & diseases molecules, & diseases, 2016-10, Vol.61, p.37-45
Hauptverfasser:	Mehta, Kosha, Busbridge, Mark, Renshaw, Derek, Evans, Robert W., Farnaud, Sebastien, Patel, Vinood B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigens, CD - drug effects Antigens, CD - genetics Hemochromatosis Protein - blood Hemochromatosis Protein - drug effects Hep G2 Cells Hepatocyte Hepcidin Hepcidins - blood Hepcidins - drug effects HepG2 Humans Iron Iron - blood Iron Overload Receptors, Transferrin - drug effects Receptors, Transferrin - genetics Recombinant Proteins RNA, Messenger - blood Telomeric Repeat Binding Protein 2 - blood Telomeric Repeat Binding Protein 2 - drug effects Time Factors Transferrin Transferrin - pharmacology
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Zusammenfassung:	Hepcidin is the key regulator of systemic iron homeostasis. The iron-sensing mechanisms and the role of intracellular iron in modulating hepatic hepcidin secretion are unclear. Therefore, we created a novel cell line, recombinant-TfR1 HepG2, expressing iron-response-element-independent TFRC mRNA to promote cellular iron-overload and examined the effect of excess holotransferrin (5g/L) on cell-surface TfR1, iron content, hepcidin secretion and mRNA expressions of TFRC, HAMP, SLC40A1, HFE and TFR2. Results showed that the recombinant cells exceeded levels of cell-surface TfR1 in wild-type cells under basal (2.8-fold; p
ISSN:	1079-9796 1096-0961
DOI:	10.1016/j.bcmd.2016.06.008