Bioconcentration of nonylphenol in fathead minnows ( Pimephales promelas)

Bioconcentration of nonylphenol in fathead minnows ( Pimephales promelas)

Bioconcentration of p-nonylphenol (NP) by fathead minnows was determined under laboratory conditions. Fish were exposed continuously for 42 days to 0.33, 0.93 and 2.36 μg NP/l in a flow-through system. NP was Soxhlet extracted from whole fish homogenates with dichloromethane (DCM). The resulting ext...

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Veröffentlicht in:Chemosphere (Oxford) 2001-09, Vol.44 (8), p.1697-1702
Hauptverfasser: Snyder, Shane A., Keith, Timothy L., Pierens, Susan L., Snyder, Erin M., Giesy, John P.
Format: Artikel
Sprache:eng
Schlagworte:
Agnatha. Pisces
Alkylphenol
Animal, plant and microbial ecology
Animals
Applied ecology
Bioaccumulation
Bioconcentration factor
Biological and medical sciences
Chromatography, High Pressure Liquid
Cyprinidae
Ecotoxicology, biological effects of pollution
Effects of pollution and side effects of pesticides on vertebrates
Electrophoresis, Gel, Two-Dimensional
Fundamental and applied biological sciences. Psychology
Lipids
Mass Spectrometry
p-Nonylphenol
Phenols - pharmacokinetics
Pimephales promelas
Surfactant
Tissue Distribution
Water Pollutants, Chemical - pharmacokinetics
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Zusammenfassung:Bioconcentration of p-nonylphenol (NP) by fathead minnows was determined under laboratory conditions. Fish were exposed continuously for 42 days to 0.33, 0.93 and 2.36 μg NP/l in a flow-through system. NP was Soxhlet extracted from whole fish homogenates with dichloromethane (DCM). The resulting extract was concentrated and bulk lipids removed by gel permeation and silica-gel chromatography. Compounds were identified and quantified by reverse-phase high-pressure liquid chromatography (RP-HPLC) with fluorescence detection. Mass spectrometry was used for verification of peak assignments. Bioconcentration factors (BCFs) ranged from 245 to 380.
ISSN:0045-6535
1879-1298
DOI:10.1016/S0045-6535(00)00524-5