Development of a Monoclonal Antibody Against Porcine Circovirus2 Cap Protein

Female BALB/c mice were immunized with a commercial PCV2 vaccine, and a monoclonal antibody (MAb) designated as 3H11 was achieved by hybridoma techniques. The MAb specifically reacted with Cap protein of PCV2, which has been identified by western blot. Immunoperoxidase monolayer assay results showed...

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Veröffentlicht in:	Monoclonal antibodies in immunodiagnosis and immunotherapy 2016-08, Vol.35 (4), p.227-230
Hauptverfasser:	Gu, Yanli, Zheng, Dingding, Jin, Yudan, Hao, Liying, Xuan, Yajie, Pang, Wenqiang, Huang, Yuxin, Li, Xiangdong, Deng, Junhua, Tian, Kegong
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - immunology Antibodies, Viral - immunology Antibody Specificity - immunology Capsid Proteins - immunology Circovirus - immunology Circovirus - pathogenicity Epitope Mapping Female Mice Mice, Inbred BALB C Original Articles Swine
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Beschreibung
Zusammenfassung:	Female BALB/c mice were immunized with a commercial PCV2 vaccine, and a monoclonal antibody (MAb) designated as 3H11 was achieved by hybridoma techniques. The MAb specifically reacted with Cap protein of PCV2, which has been identified by western blot. Immunoperoxidase monolayer assay results showed that 3H11 did not cross-react with PCV1-infected cells. Therefore, this work suggested that 3H11 could be a useful tool as a specific diagnostic reagent for PCV2 research.
ISSN:	2167-9436 2167-9436
DOI:	10.1089/mab.2016.0021