Effect of transtympanic betamethasone delivery to the inner ear
To investigate the effect of transtympanic betamethasone administration on hearing function with histologic correlation, rats were divided into three transtympanic treatment groups: isotonic saline (group I, n = 10), gentamicin (group II, n = 10) and betamethasone (group III, n = 10). Distortion...
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| creator | Özel, Halil Erdem Özdoğan, Fatih Gülşen Gürgen, Seren Esen, Erkan Selçuk, Adin Genç, Selahattin |
| description | To investigate the effect of
transtympanic
betamethasone administration on hearing function with histologic correlation, rats were divided into three
transtympanic
treatment groups: isotonic saline (group I,
n
= 10), gentamicin (group II,
n
= 10) and betamethasone (group III,
n
= 10). Distortion product otoacoustic emission thresholds were compared on day 10. Also histological effects on cellular apoptosis in both the inner and outer hair cells in organ of Corti and spiral ganglion neurons were evaluated. Distortion product otoacoustic emission thresholds were comparable (
p
> 0.05) between group I and group III in all measurements. Distortion product otoacoustic emission thresholds of group II were significantly elevated in all measurements when compared with group I (
p
|
| doi_str_mv | 10.1007/s00405-016-3905-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1818338687</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1818338687</sourcerecordid><originalsourceid>FETCH-LOGICAL-c296t-b88cb1444c41565dc23a3746b8368e4b163ab98c4f732644677d51e15eceb8443</originalsourceid><addsrcrecordid>eNp9kD1PwzAQhi0EoqXwA1hQRpaAHV9sZ0KoKh9SJRaYLce50FSJU2wHqf-eVCmMTHfSPe8r3UPINaN3jFJ5HygFmqeUiZQX41KckDkDDinITJySOS24TAGknJGLELaU0hwKfk5mmVCcMVbMycOqrtHGpK-T6I0Lcd_tjGtsUmI0HcaNCb3DpMK2-Ua_T2KfxA0mjXPoEzT-kpzVpg14dZwL8vG0el--pOu359fl4zq1WSFiWiplSwYAFlgu8spm3HAJolRcKISSCW7KQlmoJc8EgJCyyhmyHC2WCoAvyO3Uu_P914Ah6q4JFtvWOOyHoJliinMllBxRNqHW9yF4rPXON53xe82oPnjTkzc9etMHb7oYMzfH-qHssPpL_IoagWwCwnhyn-j1th-8G1_-p_UHu2d3HA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1818338687</pqid></control><display><type>article</type><title>Effect of transtympanic betamethasone delivery to the inner ear</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Özel, Halil Erdem ; Özdoğan, Fatih ; Gülşen Gürgen, Seren ; Esen, Erkan ; Selçuk, Adin ; Genç, Selahattin</creator><creatorcontrib>Özel, Halil Erdem ; Özdoğan, Fatih ; Gülşen Gürgen, Seren ; Esen, Erkan ; Selçuk, Adin ; Genç, Selahattin</creatorcontrib><description>To investigate the effect of
transtympanic
betamethasone administration on hearing function with histologic correlation, rats were divided into three
transtympanic
treatment groups: isotonic saline (group I,
n
= 10), gentamicin (group II,
n
= 10) and betamethasone (group III,
n
= 10). Distortion product otoacoustic emission thresholds were compared on day 10. Also histological effects on cellular apoptosis in both the inner and outer hair cells in organ of Corti and spiral ganglion neurons were evaluated. Distortion product otoacoustic emission thresholds were comparable (
p
> 0.05) between group I and group III in all measurements. Distortion product otoacoustic emission thresholds of group II were significantly elevated in all measurements when compared with group I (
p
< 0.05) and group III (
p
< 0.05). In the Terminal deoxynucleotidyl transferase dUTP Nick End Labelling (TUNEL), Caspase-3, Caspase-8 and Caspase-9 staining method the amount of apoptotic cells in group II were significantly elevated in all measurements compared with group I (
p
< 0.05). In the TUNEL staining method the amount of apoptotic cells in Group III were significantly elevated compared with group I in both the organ of Corti and spiral ganglion neurons (
p
< 0.05). The overall histological results revealed that the severity of cellular apoptosis caused by betamethasone was somewhere between isotonic saline and gentamicin. Transtympanic betamethasone does not affect inner ear function as measured by distortion product otoacoustic emission responses, but some increase in cellular apoptosis in the organ of Corti and spiral ganglion neurons was observed. These findings suggest that transtympanic betamethasone may have mild ototoxic effects. Further studies are needed to obtain precise results for transtympanic application of betamethasone.</description><identifier>ISSN: 0937-4477</identifier><identifier>EISSN: 1434-4726</identifier><identifier>DOI: 10.1007/s00405-016-3905-9</identifier><identifier>PMID: 26831119</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; Apoptosis - drug effects ; Betamethasone - administration & dosage ; Caspase 3 - metabolism ; Caspase 8 - metabolism ; Cochlea - drug effects ; Cochlea - enzymology ; Ear, Inner - drug effects ; Ear, Inner - enzymology ; Ear, Inner - physiology ; Gentamicins - pharmacology ; Glucocorticoids - administration & dosage ; Hair Cells, Auditory, Outer - drug effects ; Head and Neck Surgery ; In Situ Nick-End Labeling ; Male ; Medicine ; Medicine & Public Health ; Neurosurgery ; Organ of Corti - drug effects ; Otoacoustic Emissions, Spontaneous - drug effects ; Otoacoustic Emissions, Spontaneous - physiology ; Otology ; Otorhinolaryngology ; Rats ; Rats, Wistar ; Sensory Thresholds - drug effects ; Sensory Thresholds - physiology ; Spiral Ganglion - drug effects ; Tympanic Membrane</subject><ispartof>European archives of oto-rhino-laryngology, 2016-10, Vol.273 (10), p.3053-3061</ispartof><rights>Springer-Verlag Berlin Heidelberg 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c296t-b88cb1444c41565dc23a3746b8368e4b163ab98c4f732644677d51e15eceb8443</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00405-016-3905-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00405-016-3905-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26831119$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Özel, Halil Erdem</creatorcontrib><creatorcontrib>Özdoğan, Fatih</creatorcontrib><creatorcontrib>Gülşen Gürgen, Seren</creatorcontrib><creatorcontrib>Esen, Erkan</creatorcontrib><creatorcontrib>Selçuk, Adin</creatorcontrib><creatorcontrib>Genç, Selahattin</creatorcontrib><title>Effect of transtympanic betamethasone delivery to the inner ear</title><title>European archives of oto-rhino-laryngology</title><addtitle>Eur Arch Otorhinolaryngol</addtitle><addtitle>Eur Arch Otorhinolaryngol</addtitle><description>To investigate the effect of
transtympanic
betamethasone administration on hearing function with histologic correlation, rats were divided into three
transtympanic
treatment groups: isotonic saline (group I,
n
= 10), gentamicin (group II,
n
= 10) and betamethasone (group III,
n
= 10). Distortion product otoacoustic emission thresholds were compared on day 10. Also histological effects on cellular apoptosis in both the inner and outer hair cells in organ of Corti and spiral ganglion neurons were evaluated. Distortion product otoacoustic emission thresholds were comparable (
p
> 0.05) between group I and group III in all measurements. Distortion product otoacoustic emission thresholds of group II were significantly elevated in all measurements when compared with group I (
p
< 0.05) and group III (
p
< 0.05). In the Terminal deoxynucleotidyl transferase dUTP Nick End Labelling (TUNEL), Caspase-3, Caspase-8 and Caspase-9 staining method the amount of apoptotic cells in group II were significantly elevated in all measurements compared with group I (
p
< 0.05). In the TUNEL staining method the amount of apoptotic cells in Group III were significantly elevated compared with group I in both the organ of Corti and spiral ganglion neurons (
p
< 0.05). The overall histological results revealed that the severity of cellular apoptosis caused by betamethasone was somewhere between isotonic saline and gentamicin. Transtympanic betamethasone does not affect inner ear function as measured by distortion product otoacoustic emission responses, but some increase in cellular apoptosis in the organ of Corti and spiral ganglion neurons was observed. These findings suggest that transtympanic betamethasone may have mild ototoxic effects. Further studies are needed to obtain precise results for transtympanic application of betamethasone.</description><subject>Animals</subject><subject>Apoptosis - drug effects</subject><subject>Betamethasone - administration & dosage</subject><subject>Caspase 3 - metabolism</subject><subject>Caspase 8 - metabolism</subject><subject>Cochlea - drug effects</subject><subject>Cochlea - enzymology</subject><subject>Ear, Inner - drug effects</subject><subject>Ear, Inner - enzymology</subject><subject>Ear, Inner - physiology</subject><subject>Gentamicins - pharmacology</subject><subject>Glucocorticoids - administration & dosage</subject><subject>Hair Cells, Auditory, Outer - drug effects</subject><subject>Head and Neck Surgery</subject><subject>In Situ Nick-End Labeling</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Neurosurgery</subject><subject>Organ of Corti - drug effects</subject><subject>Otoacoustic Emissions, Spontaneous - drug effects</subject><subject>Otoacoustic Emissions, Spontaneous - physiology</subject><subject>Otology</subject><subject>Otorhinolaryngology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Sensory Thresholds - drug effects</subject><subject>Sensory Thresholds - physiology</subject><subject>Spiral Ganglion - drug effects</subject><subject>Tympanic Membrane</subject><issn>0937-4477</issn><issn>1434-4726</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kD1PwzAQhi0EoqXwA1hQRpaAHV9sZ0KoKh9SJRaYLce50FSJU2wHqf-eVCmMTHfSPe8r3UPINaN3jFJ5HygFmqeUiZQX41KckDkDDinITJySOS24TAGknJGLELaU0hwKfk5mmVCcMVbMycOqrtHGpK-T6I0Lcd_tjGtsUmI0HcaNCb3DpMK2-Ua_T2KfxA0mjXPoEzT-kpzVpg14dZwL8vG0el--pOu359fl4zq1WSFiWiplSwYAFlgu8spm3HAJolRcKISSCW7KQlmoJc8EgJCyyhmyHC2WCoAvyO3Uu_P914Ah6q4JFtvWOOyHoJliinMllBxRNqHW9yF4rPXON53xe82oPnjTkzc9etMHb7oYMzfH-qHssPpL_IoagWwCwnhyn-j1th-8G1_-p_UHu2d3HA</recordid><startdate>20161001</startdate><enddate>20161001</enddate><creator>Özel, Halil Erdem</creator><creator>Özdoğan, Fatih</creator><creator>Gülşen Gürgen, Seren</creator><creator>Esen, Erkan</creator><creator>Selçuk, Adin</creator><creator>Genç, Selahattin</creator><general>Springer Berlin Heidelberg</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20161001</creationdate><title>Effect of transtympanic betamethasone delivery to the inner ear</title><author>Özel, Halil Erdem ; Özdoğan, Fatih ; Gülşen Gürgen, Seren ; Esen, Erkan ; Selçuk, Adin ; Genç, Selahattin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c296t-b88cb1444c41565dc23a3746b8368e4b163ab98c4f732644677d51e15eceb8443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Apoptosis - drug effects</topic><topic>Betamethasone - administration & dosage</topic><topic>Caspase 3 - metabolism</topic><topic>Caspase 8 - metabolism</topic><topic>Cochlea - drug effects</topic><topic>Cochlea - enzymology</topic><topic>Ear, Inner - drug effects</topic><topic>Ear, Inner - enzymology</topic><topic>Ear, Inner - physiology</topic><topic>Gentamicins - pharmacology</topic><topic>Glucocorticoids - administration & dosage</topic><topic>Hair Cells, Auditory, Outer - drug effects</topic><topic>Head and Neck Surgery</topic><topic>In Situ Nick-End Labeling</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Neurosurgery</topic><topic>Organ of Corti - drug effects</topic><topic>Otoacoustic Emissions, Spontaneous - drug effects</topic><topic>Otoacoustic Emissions, Spontaneous - physiology</topic><topic>Otology</topic><topic>Otorhinolaryngology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Sensory Thresholds - drug effects</topic><topic>Sensory Thresholds - physiology</topic><topic>Spiral Ganglion - drug effects</topic><topic>Tympanic Membrane</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Özel, Halil Erdem</creatorcontrib><creatorcontrib>Özdoğan, Fatih</creatorcontrib><creatorcontrib>Gülşen Gürgen, Seren</creatorcontrib><creatorcontrib>Esen, Erkan</creatorcontrib><creatorcontrib>Selçuk, Adin</creatorcontrib><creatorcontrib>Genç, Selahattin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European archives of oto-rhino-laryngology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Özel, Halil Erdem</au><au>Özdoğan, Fatih</au><au>Gülşen Gürgen, Seren</au><au>Esen, Erkan</au><au>Selçuk, Adin</au><au>Genç, Selahattin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of transtympanic betamethasone delivery to the inner ear</atitle><jtitle>European archives of oto-rhino-laryngology</jtitle><stitle>Eur Arch Otorhinolaryngol</stitle><addtitle>Eur Arch Otorhinolaryngol</addtitle><date>2016-10-01</date><risdate>2016</risdate><volume>273</volume><issue>10</issue><spage>3053</spage><epage>3061</epage><pages>3053-3061</pages><issn>0937-4477</issn><eissn>1434-4726</eissn><abstract>To investigate the effect of
transtympanic
betamethasone administration on hearing function with histologic correlation, rats were divided into three
transtympanic
treatment groups: isotonic saline (group I,
n
= 10), gentamicin (group II,
n
= 10) and betamethasone (group III,
n
= 10). Distortion product otoacoustic emission thresholds were compared on day 10. Also histological effects on cellular apoptosis in both the inner and outer hair cells in organ of Corti and spiral ganglion neurons were evaluated. Distortion product otoacoustic emission thresholds were comparable (
p
> 0.05) between group I and group III in all measurements. Distortion product otoacoustic emission thresholds of group II were significantly elevated in all measurements when compared with group I (
p
< 0.05) and group III (
p
< 0.05). In the Terminal deoxynucleotidyl transferase dUTP Nick End Labelling (TUNEL), Caspase-3, Caspase-8 and Caspase-9 staining method the amount of apoptotic cells in group II were significantly elevated in all measurements compared with group I (
p
< 0.05). In the TUNEL staining method the amount of apoptotic cells in Group III were significantly elevated compared with group I in both the organ of Corti and spiral ganglion neurons (
p
< 0.05). The overall histological results revealed that the severity of cellular apoptosis caused by betamethasone was somewhere between isotonic saline and gentamicin. Transtympanic betamethasone does not affect inner ear function as measured by distortion product otoacoustic emission responses, but some increase in cellular apoptosis in the organ of Corti and spiral ganglion neurons was observed. These findings suggest that transtympanic betamethasone may have mild ototoxic effects. Further studies are needed to obtain precise results for transtympanic application of betamethasone.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>26831119</pmid><doi>10.1007/s00405-016-3905-9</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 0937-4477 |
| ispartof | European archives of oto-rhino-laryngology, 2016-10, Vol.273 (10), p.3053-3061 |
| issn | 0937-4477 1434-4726 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1818338687 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Animals Apoptosis - drug effects Betamethasone - administration & dosage Caspase 3 - metabolism Caspase 8 - metabolism Cochlea - drug effects Cochlea - enzymology Ear, Inner - drug effects Ear, Inner - enzymology Ear, Inner - physiology Gentamicins - pharmacology Glucocorticoids - administration & dosage Hair Cells, Auditory, Outer - drug effects Head and Neck Surgery In Situ Nick-End Labeling Male Medicine Medicine & Public Health Neurosurgery Organ of Corti - drug effects Otoacoustic Emissions, Spontaneous - drug effects Otoacoustic Emissions, Spontaneous - physiology Otology Otorhinolaryngology Rats Rats, Wistar Sensory Thresholds - drug effects Sensory Thresholds - physiology Spiral Ganglion - drug effects Tympanic Membrane |
| title | Effect of transtympanic betamethasone delivery to the inner ear |
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