Feasibility analysis of quantitative detection on serum HBeAg/HBeAb by time-resolved immunofluorescence assay

Feasibility analysis of quantitative detection on serum HBeAg/HBeAb by time-resolved immunofluorescence assay

To determine whether time-resolved immunofluorescence assay (TRIFA) shares the similar accuracy and specificity with automatic chemiluminescence immunoassay (CMIA) in analyzing HBeAg levels in hepatitis B.
 A total of 157 serum samples were collected from outpatients with infection of HBV in Xiangya...

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Veröffentlicht in:Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban 2016-08, Vol.41 (8), p.852-855
Hauptverfasser: Fu, Xiaoyu, Wu, Feiyuan, Chen, Gang, Xie, Yanling, Deng, Guohua, Gan, Shaojun, Fu, Lei
Format: Artikel
Sprache:chi
Schlagworte:
Feasibility Studies
Fluorescent Antibody Technique
Hepatitis B
Hepatitis B Antibodies
Hepatitis B e Antigens
Hepatitis B virus
Humans
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Zusammenfassung:To determine whether time-resolved immunofluorescence assay (TRIFA) shares the similar accuracy and specificity with automatic chemiluminescence immunoassay (CMIA) in analyzing HBeAg levels in hepatitis B.
 A total of 157 serum samples were collected from outpatients with infection of HBV in Xiangya Hospital, Central South University. CMIA and TRIFA were used to analyze HBeAg quantitation and HBeAg/HBeAb qualitative detection, respectively.
 The linear regression equation for the two methods was Y=0.72779X-4.0551 (r=0.712, P
ISSN:1672-7347
DOI:10.11817/j.issn.1672-7347.2016.08.013