Identification and gene cloning of a new major allergen Cha o 3 from Chamaecyparis obtusa (Japanese cypress) pollen

The Cha o 3 double bands were periodic acid-Schiff-stain positive, and the same peptide fragments were detected by LC-MS/MS from digests of each band (data not shown). [...]we concluded that the Cha o 3 double bands were the result of glycosylation difference, as with Cha o 1.3 To confirm whether Cha o 3 is an allergen in humans, an ELISA was performed to detect Cha o 3-specific IgE in the serum of 16 patients with Japanese cypress pollinosis. [...]to confirm the cross-reactivity of Cha o 3 with Japanese cedar allergen, we evaluated the production of IL-5, a typical TH2 cytokine, in PBMCs isolated from another panel of patients with Japanese cedar pollinosis, with and without Japanese cedar-specific immunotherapy.