Spent grains: a new support for brewing yeast immobilisation

A novel carrier obtained from spent grains, a brewing by-product, was used for brewing yeast immobilisation in a continuous bubble-column reactor. The multiple-layer cell adhesion to the carrier particles resulted in a maximum cell load of 430 mg dry cell gˉ¹ dry carrier (d.c.). After 120 h of react...

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Veröffentlicht in:	Biotechnology letters 2001-01, Vol.23 (13), p.1073-1078
Hauptverfasser:	Brányik, Tomáš, Vicente, A. A., Cruz, José Machado, Teixeira, J. A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Beers Biological and medical sciences Biotechnology Cell adhesion & migration Continuous reactor Fermented food industries Food industries Fundamental and applied biological sciences. Psychology Immobilisation Immobilization of organelles and whole cells Immobilization techniques Methods. Procedures. Technologies Reactors Science & Technology Spent grains Yeast Yeasts
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Beschreibung
Zusammenfassung:	A novel carrier obtained from spent grains, a brewing by-product, was used for brewing yeast immobilisation in a continuous bubble-column reactor. The multiple-layer cell adhesion to the carrier particles resulted in a maximum cell load of 430 mg dry cell gˉ¹ dry carrier (d.c.). After 120 h of reactor operation, the cell load of DEAEmodified carrier was below 40 mg dry cell gˉ¹ d.c. while the values for non-modified carrier reached at least 100 mg dry cell gˉ¹ d.c. The changes in substrate composition on the rate of yeast attachment and on its stability were also studied.
ISSN:	0141-5492 1573-6776
DOI:	10.1023/A:1010558407475