Dextran sulfate sodium-induced acute experimental colitis in C57BL/6 mice is mitigated by selenium
Sodium selenite has been shown to have a protective role in experimental colitis. Th1 and Th17 responses are involved in the pathogenesis of dextran sulfate sodium (DSS)-induced colitis and inflammatory bowel disease. This study investigated whether sodium selenite can suppress Th1/Th17-mediated exp...
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Veröffentlicht in: | International immunopharmacology 2016-10, Vol.39, p.359-368 |
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Sprache: | eng |
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Zusammenfassung: | Sodium selenite has been shown to have a protective role in experimental colitis. Th1 and Th17 responses are involved in the pathogenesis of dextran sulfate sodium (DSS)-induced colitis and inflammatory bowel disease. This study investigated whether sodium selenite can suppress Th1/Th17-mediated experimental colitis.
Mice were administered sodium selenite (2μg/g body weight) by gavage daily for 30days. Beginning on day 21, mice were administered 2.5% oral DSS for 9days. The mice were sacrificed on day 31. Survival rates, clinical symptoms, colon lengths, and histological changes were determined.
Pretreatment with sodium selenite (2μg/g body weight) improved survival rates, colon shortening, body weight loss, disease activity index, and histopathological score in mice with DSS-induced colitis. Pretreatment with sodium selenite restored interleukin-10 and Foxp3 excretion, as well as reducing the levels of interferon-γ and interleukin-17A.
Pretreatment with sodium selenite showed therapeutic potential for preventing colitis in mice. This effect may be mediated by the immunomodulation of regulatory T cells, expressing anti-inflammatory genes that suppress Th1 and Th17 responses.
•Selenium pretreatment significantly attenuated the experimental colitis.•Selenium pretreatment restored interleukin-10 excretion, as well as reducing the levels of interferon-γ and interleukin-17A.•Selenium corrected DSS-induced imbalance of Th1-Th17/Treg cells. |
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ISSN: | 1567-5769 1878-1705 |
DOI: | 10.1016/j.intimp.2016.07.034 |