The essential role of inorganic substrate in the migration and osteoblastic differentiation of mesenchymal stem cells

The physical environment, which is an integral part of the stem cell niche, is critical in regulating stem cell functions and differentiation into specific lineages. Previous studies have primarily focused on modulating the polymeric matrixes, including the extracellular matrix. Here, we report that the presence of the inorganic substrate (Ti and hydroxyapatite (HA)) in addition to the collagen overlayer plays an essential role in cytoskeletal organization, migration and differentiation of mesenchymal stem cells (MSCs). The osteogenic differentiation of MSCs was suppressed on pure collagen substrate alone, despite collagen greatly enhancing the MSC adhesion and proliferation. The results indicated a strong correlation between MSC motility and osteoblastic differentiation. In particular, the presence of the inorganic matrix promoted the activation of the canonical WNT-β-Catenin pathway and stimulated transcription, leading to osteoblastic differentiation, which was likely due to the internal forces generated “dynamically” during cell migration. Compared to the Ti substrate, hydroxyapatite promoted the collagen self-assembly and the formation of the collagen fibrous network, which is critical for MSC motility and osteogenic differentiation. The HA-collagen matrix exhibited the most favourable stress fibre formation, the longest migration distance (2.8-fold higher than that of the pure collagen sample and 1.9-fold higher than that of Ti-collagen), and the best osteogenic differentiation activities. These findings might have important implications for our understanding of the fundamental MSC functions and the optimal design of bone regeneration materials. ▪ •Collagen enhances MSC adhesion and proliferation, but inhibits OB differentiation.•The inorganic matrix modulates the cytoskeleton organization and migration.•A strong correlation between the MSC motility with the OB differentiation.•Inorganic matrix activates of WNT pathway, stimulating OB differentiation.