Sonodynamic therapy using 5-aminolevulinic acid enhances the efficacy of bleomycin
•Efficacy of sonodynamic therapy (SDT) with bleomycin was studied in EMT-6 cells.•SDT with bleomycin was more cytotoxic than SDT alone.•In vivo, SDT with bleomycin achieved greater tumor growth inhibition than SDT alone.•Bleomycin improved the efficacy of SDT.•SDT with bleomycin might be a noninvasi...
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Veröffentlicht in: | Ultrasonics 2016-04, Vol.67, p.76-84 |
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Sprache: | eng |
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Zusammenfassung: | •Efficacy of sonodynamic therapy (SDT) with bleomycin was studied in EMT-6 cells.•SDT with bleomycin was more cytotoxic than SDT alone.•In vivo, SDT with bleomycin achieved greater tumor growth inhibition than SDT alone.•Bleomycin improved the efficacy of SDT.•SDT with bleomycin might be a noninvasive treatment for deep-seated tumors.
Sonodynamic therapy (SDT) kills tumor cells through the synergistic effects of ultrasound and a sonosensitizer agent. We examined whether 5-aminolevulinic acid (5-ALA)-based SDT at 1 or 3MHz could enhance the cytotoxicity of bleomycin (BLM) toward mouse mammary tumor cells both in vitro and in vivo. At 1MHz, cell viability in the 5-ALA-based SDT group at 1, 2, and 3W/cm2 was 34.30%, 50.90%, and 60.16%, respectively. Cell viability in the 5-ALA-based SDT+BLM group at 1, 2, and 3W/cm2 was 0.09%, 0.32%, and 0.17%, respectively. In contrast, at 3MHz, 5-ALA-based SDT+BLM did not show pronounced cytotoxicity. In the in vivo study, 5-ALA-based SDT+BLM was significantly more cytotoxic than 5-ALA-based SDT at 1MHz and 3MHz. These findings suggest that the mechanism of tumor shrinkage induced by 5-ALA-based SDT+BLM might involve not only direct cell killing, but also vascular shutdown. Thus, we show here that 5-ALA-based SDT enhances the efficacy of BLM both in vitro and in vivo. |
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ISSN: | 0041-624X 1874-9968 |
DOI: | 10.1016/j.ultras.2016.01.003 |