Bioelectrocatalytic Oxygen Reaction and Chloride Inhibition Resistance of Laccase Immobilized on Single-walled Carbon Nanotube and Carbon Paper Electrodes

The rate of heterogeneous direct electron transfer of laccase immobilized on single-walled carbon nanotube (SWCNT) and carbon paper electrodes was evaluated by cyclic voltammetry and background-current-corrected steady-state linear voltammetry. These rates indicated that the molecular orientation of laccase immobilized on the SWCNT electrode was more favorable for direct electron transfer, than that of laccase immobilized on the carbon paper electrode. The inhibition of the bioelectrocatalytic O2 reduction current of the two electrodes by chloride and fluoride were tested. The results indicated differing inhibition mechanisms by these two halides. Laccase immobilized on the SWCNT electrode exhibited high stability and high resistance to chloride inhibition.