Hairpin DNA Unzipping Analysis Using a Biological Nanopore Array

Hairpin DNA Unzipping Analysis Using a Biological Nanopore Array

This paper describes a method of hairpin DNA (hpDNA) unzipping analysis using a biological nanopore array. Various lengths of hpDNAs were captured and translocated through the nanopore and the individual duration times were monitored. The correlation between the unzipping time and the simulated hybr...

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Veröffentlicht in:Denki kagaku oyobi kōgyō butsuri kagaku 2016-01, Vol.84 (5), p.338-338
Hauptverfasser: OHARA, Masayuki, SEKIYA, Yusuke, KAWANO, Ryuji
Format: Artikel
Sprache:jpn
Schlagworte:
Arrays
Biological
Computer simulation
Correlation
Deoxyribonucleic acid
Mathematical models
Nanostructure
Replication
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Zusammenfassung:This paper describes a method of hairpin DNA (hpDNA) unzipping analysis using a biological nanopore array. Various lengths of hpDNAs were captured and translocated through the nanopore and the individual duration times were monitored. The correlation between the unzipping time and the simulated hybridization energy of the duplexes was able to be modeled as first-ordered reaction. This method is one of the approach for understanding of basic biological reactions in DNA replication and RNA transcription.
ISSN:1344-3542