OsSNAP32, a SNAP25-type SNARE protein-encoding gene from rice, enhanced resistance to blast fungus

SNAREs play important roles in plant responses to various biotic and abiotic stresses. OsSNAP32 is a SNAP25-type SNARE protein-encoding gene isolated from rice. OsSNAP32 is ubiquitously expressed in the various tissues of blast-resistant rice landrace Heikezijing and blast-susceptible Suyunuo, with higher expression in Heikezijing and induced expression in rice seedlings inoculated by the blast pathogen ( Magnaporthe oryzae ) race Hoku1. OsSNAP32 -overexpressing transgenic lines in Suyunuo increased resistance to blast, with fewer lesions in the inoculated leaves compared with the non-transgenic control Suyunuo. OsSNAP32 RNAi transgenic lines in Heikezijing decreased resistance to blast, with some typical lesions in the inoculated leaves compared with the non-transgenic control Heikezijing. These results suggested that OsSNAP32 might be involved in rice resistance to blast.