High genetic diversity but low population structure in the frog Pseudopaludicola falcipes (Hensel, 1867) (Amphibia, Anura) from the Pampas of South America

[Display omitted] •The frog Pseudopaludicola falcipes is a single species distributed in the Pampas.•There are two populations: one in Uruguay and Argentina, and the other in Brazil.•Both populations show poor geographic structure and large effective sizes.•These populations diverged, grew, and exchanged migrants during the Pleistocene.•The dispersal history appears associated with interglacial phases of the Quaternary.•Our study is the first phylogeographic analysis of a frog species from the Pampas. Relative to South America’s ecoregions, the temperate grasslands of the Pampas have been poorly studied from a phylogeographic perspective. Based on an intermediate biogeographic setting between subtropical forest (Atlantic Forest) and arid ecosystems (Chaco and Patagonia), Pampean species are expected to show unstable demographic histories due to the Quaternary climatic oscillations. Herein, we investigate the phylogenetic relatedness and phylogeographic history of Pseudopaludicola falcipes, a small and common frog that is widely distributed across the Pampean grasslands. First, we use molecular data to assess if P. falcipes represents a single or multiple, separately evolving cryptic lineages. Because P. falcipes is a small-size species (