Active biomonitoring of mussels Mytilus galloprovincialis with integrated use of micronucleus assay and physiological indices to assess harbor pollution

The mussels Mytilus galloprovincialis collected from a noncontaminated site (Chaib Rasso) were transplanted during one, three and six months at Ghazaouet harbor (GH), areas with a strong gradient of pollution. The micronucleus test (MN) was selected to monitor the impact of contamination, along with physiological indexes (condition index CI and organo-somatic indexes RI and GSI). The results show a negative correlation of MN variation in gill cells with CI but a positive correlation with transplantation duration. However, a significant correlation was found between the indexes. Moreover, the findings indicate that MN in the hemolymph and gills of transplanted mussels for one, three and six months at GH are significantly higher than those of the reference site. However, no significant differences were noted between the three transplants at the two organs. Monitoring the physiological status of mussels, in parallel with the biomarker measurements, is useful in assessing the impact of contaminants. •MN test is useful in active biomonitoring.•Monitoring the MN test and physiological indexes complement the information on mussels' status.•A long duration of transplantation allows to follow the impact of pollution on the physiology of animal.•Ghazaouet harbor is designate as contaminated area compared to reference site.•Gills are an excellent tissue to monitor the impact of contamination in mussels using MN test.