An evaluation of transcriptome-based exon capture for frog phylogenomics across multiple scales of divergence (Class: Amphibia, Order: Anura)

Custom sequence capture experiments are becoming an efficient approach for gathering large sets of orthologous markers in nonmodel organisms. Transcriptome‐based exon capture utilizes transcript sequences to design capture probes, typically using a reference genome to identify intron–exon boundaries...

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Veröffentlicht in:	Molecular ecology resources 2016-09, Vol.16 (5), p.1069-1083
Hauptverfasser:	Portik, Daniel M., Smith, Lydia L., Bi, Ke
Format:	Artikel
Sprache:	eng
Schlagworte:	Amphibia amphibians Animals Anura Anura - classification Anura - genetics DNA - chemistry DNA - genetics DNA - isolation & purification Evolution, Molecular Exons genomics Genotyping Techniques - methods High-Throughput Nucleotide Sequencing Nucleic Acid Hybridization - methods Oligonucleotide Probes - genetics phylogenomics Phylogeny sequence capture Specimen Handling - methods Transcriptome transcriptome-based exon capture
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Beschreibung
Zusammenfassung:	Custom sequence capture experiments are becoming an efficient approach for gathering large sets of orthologous markers in nonmodel organisms. Transcriptome‐based exon capture utilizes transcript sequences to design capture probes, typically using a reference genome to identify intron–exon boundaries to exclude shorter exons (
ISSN:	1755-098X 1755-0998
DOI:	10.1111/1755-0998.12541