High-Throughput Sequencing Reveals Immunological Characteristics of the TRB-/IgH-CDR3 Region of Umbilical Cord Blood

Objective To compare the differences of immunological characteristics between newborn and adults, we performed high-throughput sequencing to reveal the diversity of umbilical cord blood and adult peripheral blood at both T-cell receptor beta chain (TRB) and immunoglobulin heavy chain (IGH) levels. S...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	The Journal of pediatrics 2016-09, Vol.176, p.69-78.e1
Hauptverfasser:	Guo, Changlong, MD, PhD, Wang, Qidi, MD, PhD, Cao, Xiaofang, MD, Yang, Ying, MD, PhD, Liu, Xin, PhD, An, Lisha, MD, Cai, Ruikun, PhD, Du, Meng, MD, Wang, Guangyu, MD, Qiu, Yue, MD, Peng, Zuoqi, MD, Han, Jian, MD, PhD, Ni, Shuhua, PhD, Tan, Xuerui, MD, PhD, Jin, Li, MD, Yu, Song, MD, Wang, Huiying, MD, Wang, Chunlin, PhD, Wang, Xingyu, MD, PhD, Ma, Xu, MD, PhD
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Complementarity Determining Regions - blood Complementarity Determining Regions - genetics Complementarity Determining Regions - immunology Female Fetal Blood High-Throughput Nucleotide Sequencing high-throughput sequencing Humans Immunoglobulin Heavy Chains - blood Immunoglobulin Heavy Chains - genetics Immunoglobulin Heavy Chains - immunology Infant, Newborn Pediatrics Pregnancy Receptors, Antigen, T-Cell, alpha-beta - blood Receptors, Antigen, T-Cell, alpha-beta - genetics Receptors, Antigen, T-Cell, alpha-beta - immunology Sequence Analysis, DNA TRB-/IgH-CDR3 repertoires umbilical cord blood
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Objective To compare the differences of immunological characteristics between newborn and adults, we performed high-throughput sequencing to reveal the diversity of umbilical cord blood and adult peripheral blood at both T-cell receptor beta chain (TRB) and immunoglobulin heavy chain (IGH) levels. Study design High-throughput sequencing was performed to analyze the expression of TRB-CDR3 and IGH-CDR3 in circulating T and B cells isolated from 20 healthy adults, 56 pregnant women, and 40 newborns. Results Our results revealed different immunological characteristics between newborn and adults, such as distinctive complementarity determining region 3 (CDR3) lengths, usage bias of variable and joining segments, random nucleotide addition, a large number of unique CDR3 peptides, and a greater repertoire diversity. Moreover, each newborn had a distinctive TRB-/IGH-CDR3 repertoire that was independent of the maternal immune status. Conclusions This study presents comprehensive, unrestricted profiles of the TRB/IGH-CDR3 repertoire of newborns, pregnant women, and healthy adults at a sequence-level resolution. Our data may contribute to a better understanding of the immune system of newborns and benefit the efficient application of umbilical cord blood transplantation in future.
ISSN:	0022-3476 1097-6833
DOI:	10.1016/j.jpeds.2016.05.078