Recent insights on applications of pullulan in tissue engineering

•Recent insights of applications of pullulan in tissue engineering have been discussed.•Pullulan as a scaffold for tissue engineering has been described.•Imperative role of pullulan in bone tissue engineering has been elaborated.•Pullulan applications for small and full thickness wound healing have been explained.•Vascular endothelial cells regeneration using pullulan composite scaffolds has been enlightened. Tissue engineering is a recently emerging line of act which assists the regeneration of damaged tissues, unable to self-repair themselves and in turn, enhances the natural healing potential of patients. The repair of injured tissue can be induced with the help of some artificially created polymer scaffolds for successful tissue regeneration. The pullulan composite scaffolds can be used to enhance the proliferation and differentiation of cells for tissue regeneration. The unique pattern of pullulan with α-(1→4) and α-(1→6) linkages along with the presence of nine hydroxyl groups on its surface, endows the polymer with distinctive physical features required for tissue engineering. Pullulan can be used for vascular engineering, bone repair and skin tissue engineering. Pullulan composite scaffolds can also be used for treatment of injured femoral condyle bone, skull bone and full thickness skin wound of murine models, transversal mandibular and tibial osteotomy in goat, etc. This review article highlights the latest developments on applications of pullulan and its derivatives in tissue engineering.