Depletion of autophagy receptor p62/SQSTM1 enhances the efficiency of gene delivery in mammalian cells

Depletion of autophagy receptor p62/SQSTM1 enhances the efficiency of gene delivery in mammalian cells

Novel methods that increase the efficiency of gene delivery to cells will have many useful applications. Here, we report a simple approach involving depletion of p62/SQSTM1 to enhance the efficiency of gene delivery. The efficiency of reporter gene delivery was remarkably higher in p62‐knockout muri...

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Veröffentlicht in:FEBS letters 2016-08, Vol.590 (16), p.2671-2680
Hauptverfasser: Tsuchiya, Megumi, Ogawa, Hidesato, Koujin, Takako, Kobayashi, Shouhei, Mori, Chie, Hiraoka, Yasushi, Haraguchi, Tokuko
Format: Artikel
Sprache:eng
Schlagworte:
Animals
autophagy
DNA beads
Fibroblasts - metabolism
Gene Knockout Techniques
Gene Transfer Techniques
Genetic Therapy
HeLa Cells
Humans
LC3
Mice
Mouse Embryonic Stem Cells - metabolism
RNA, Small Interfering - genetics
Sequestosome-1 Protein - antagonists & inhibitors
Sequestosome-1 Protein - genetics
Transfection - methods
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Zusammenfassung:Novel methods that increase the efficiency of gene delivery to cells will have many useful applications. Here, we report a simple approach involving depletion of p62/SQSTM1 to enhance the efficiency of gene delivery. The efficiency of reporter gene delivery was remarkably higher in p62‐knockout murine embryonic fibroblast (MEF) cells compared with normal MEF cells. This higher efficiency was partially attenuated by ectopic expression of p62. Furthermore, siRNA‐mediated knockdown of p62 clearly increased the efficiency of transfection of murine embryonic stem (mES) cells and human HeLa cells. These data indicate that p62 acts as a key regulator of gene delivery.
ISSN:0014-5793
1873-3468
DOI:10.1002/1873-3468.12262