Lead sensors development and antimicrobial activities based on graphene oxide/carbon nanotube/poly(O-toluidine) nanocomposite

[Display omitted] •We chemically synthesized GO-CNT-POT nanocomposite was prepared by a chemical polymerization process and characterized by XRD and FESEM analysis and show an evidence of GO-CNT-POT nanocomposites.•The antibacterial study showed that the GO-CNT-POT nanocomposite was found to be most effective against both B. subtilis and E. coli respectively which was significant compared to the amoxicillin.•Thin-layer of GO-CNT-POT nanocomposite onto glassy carbon electrode (GCE) is fabricated with conducting coating agents to fabricate a sensitive and selective Pb2+ ions sensor in short response time in phosphate buffer phase.•The sensitivity and detection limit is ∼8.53164μAμM−1cm−2 and ∼89.0 pM (signal-to-noise ratio, at an SNR of 3) respectively. Graphene oxide/carbon nanotube/poly (O-toluidine) (GO-CNT-POT) nanocomposite was prepared by a situ polymerization method and characterized by X-ray powder diffractometry (XRD) and scanning electron microscopy (SEM). The antibacterial activity of the obtained GO-CNT-POT nanocomposite was also evaluated against Gram positive bacteria Bacillus subtilis, Gram negative bacteria Escherichia coli and antibiotics (Amoxicillin) using the agar plate. The antibacterial study showed that the GO-CNT-POT was found to be most effective against both B. subtilis and E. coli respectively which was significant compared to the amoxicillin and the simultaneously GO-CNT-POT nanocomposite were fabricated onto glassy carbon electrode (GCE) using conducting coating binders by I–V technique, where the total analytical parameters were measured for the development of sensitive lead sensors (Pb2+). The GO-CNT-POT nanocomposite were deposited on flat-GCE (surface area: ∼0.0316cm2) to result in a sensor that has a fast response to selective Pb2+ ions in buffer system. Features including sensitivity, detection limit, reproducibility, linear dynamic range, selectivity, and electrochemical performances were investigated in details with the GO-CNT-POT nanocomposite fabricated GCE electrodes. The calibration plot is linear (r2: 0.9907) over the large concentration range (0.1nM to 1.0mM). The sensitivity and detection limit is calculated as 8.53164μAcm−2μM−1 and 89.0 pM (at a signal-to-noise-ratio, SNR of 3) respectively.