A carbon dot-hemoglobin complex-based biosensor for cholesterol detection
In the present study, a carbon dot/hemoglobin (CD/Hb) complex is used as a bio-receptor in an optical cholesterol biosensor. This optical sensor detects cholesterol through fluorescence enhancement of CD, which is normally quenched via π-π interactions between CD and Hb in the CD/Hb complex. CD is r...
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Veröffentlicht in: | Green chemistry : an international journal and green chemistry resource : GC 2016, Vol.18 (15), p.4245-4253 |
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Sprache: | eng |
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Zusammenfassung: | In the present study, a carbon dot/hemoglobin (CD/Hb) complex is used as a bio-receptor in an optical cholesterol biosensor. This optical sensor detects cholesterol through fluorescence enhancement of CD, which is normally quenched
via
π-π interactions between CD and Hb in the CD/Hb complex. CD is released from the CD/Hb complex because hydrophobic interactions between Hb and cholesterol are more favorable than π-π interactions between CD and Hb. The CD/Hb complex enabled selective detection of cholesterol within a linear range from 0 to 800 μM, with a limit of detection of 56 μM and a response time of ≤5 minutes in human blood plasma. Compared with other cholesterol sensors, a CD/Hb complex-based biosensor is simple, highly sensitive, selective, rapid, eco-friendly, and cost-effective for cholesterol detection in both biological and environmental samples.
In the present study, a carbon dot/hemoglobin (CD/Hb) complex is used as a bio-receptor in an optical cholesterol biosensor. |
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ISSN: | 1463-9262 1463-9270 |
DOI: | 10.1039/c6gc00507a |