A carbon dot-hemoglobin complex-based biosensor for cholesterol detection

In the present study, a carbon dot/hemoglobin (CD/Hb) complex is used as a bio-receptor in an optical cholesterol biosensor. This optical sensor detects cholesterol through fluorescence enhancement of CD, which is normally quenched via π-π interactions between CD and Hb in the CD/Hb complex. CD is r...

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Veröffentlicht in:Green chemistry : an international journal and green chemistry resource : GC 2016, Vol.18 (15), p.4245-4253
Hauptverfasser: Bui, Trang Thi, Park, Soo-Young
Format: Artikel
Sprache:eng
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Zusammenfassung:In the present study, a carbon dot/hemoglobin (CD/Hb) complex is used as a bio-receptor in an optical cholesterol biosensor. This optical sensor detects cholesterol through fluorescence enhancement of CD, which is normally quenched via π-π interactions between CD and Hb in the CD/Hb complex. CD is released from the CD/Hb complex because hydrophobic interactions between Hb and cholesterol are more favorable than π-π interactions between CD and Hb. The CD/Hb complex enabled selective detection of cholesterol within a linear range from 0 to 800 μM, with a limit of detection of 56 μM and a response time of ≤5 minutes in human blood plasma. Compared with other cholesterol sensors, a CD/Hb complex-based biosensor is simple, highly sensitive, selective, rapid, eco-friendly, and cost-effective for cholesterol detection in both biological and environmental samples. In the present study, a carbon dot/hemoglobin (CD/Hb) complex is used as a bio-receptor in an optical cholesterol biosensor.
ISSN:1463-9262
1463-9270
DOI:10.1039/c6gc00507a