Exogenous Gene Expression on Transgenic Populus x euramericana cv. '74/76'Carrying Bivalent Insect-Resistant Genes

[Objective]In order to identify and analyze the expression of exogenous genes in transgenic Populus x euramericana cv. '74/76 ' carrying bivalent insect-resistant genes BtCry1Ac and API ( Abbreviation: Transgenic 107 poplar) ,and to select transgenic 107 poplar lines with high resistance to Lepidoptera pests Hlyphantria cunea and Clostera anachoreta. [Method]Two-year-old field seedlings of ten transgenic 107 poplar lines were selected as materials and non-transgenic 107 poplar as control. Detections of exogenous gene expression were conducted,including PCR detection, absolute fluorescence quantitative PCR,toxin detection and comparative studies on insect resistance of Lepidoptera pests.[Result]PCR detection showed that exogenous genes BtCry1Ac and API existed in the ten transgenic lines,but not in the CK,indicating that BtCry1Ac and API were steadily inserted into transgenic plant genome. The fluorescence quantitative PCR showed transcription abundance of BtCry1Ac in five lines PB1,PB2,PB6,PB9,and PB10 were h