Preclinical platform of retinoblastoma xenografts recapitulating human disease and molecular markers of dissemination

Highlights • Preclinical retinoblastoma models derived from heavily pretreated patients are scarce. • Novel patient-derived preclinical retinoblastoma models (cell cultures in serum-free medium and orthotopic xenografts) reproduce key histologic and genetic features of paired patient biopsies. • Mol...

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Veröffentlicht in:	Cancer letters 2016-09, Vol.380 (1), p.10-19
Hauptverfasser:	Pascual-Pasto, Guillem, Olaciregui, Nagore G, Vila-Ubach, Monica, Paco, Sonia, Monterrubio, Carles, Rodriguez, Eva, Winter, Ursula, Batalla-Vilacis, Mireia, Catala, Jaume, Salvador, Hector, Parareda, Andreu, Schaiquevich, Paula, Suñol, Mariona, Mora, Jaume, Lavarino, Cinzia, de Torres, Carmen, Chantada, Guillermo L, Carcaboso, Angel M
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Biopsy Brain Neoplasms - enzymology Brain Neoplasms - genetics Brain Neoplasms - secondary Cell culture Cell Line, Tumor Cell Movement Chemotherapy Child, Preschool Conflicts of interest CRX Deoxyribonucleic acid DNA Female Gene expression Gene Expression Regulation, Enzymologic Gene Expression Regulation, Neoplastic Genomes Hematology, Oncology and Palliative Medicine Heterografts Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Humans Infant Laboratories Metastasis Mice, Nude Mutation N-Acetylgalactosaminyltransferases - genetics N-Acetylgalactosaminyltransferases - metabolism Neoplasm Micrometastasis Neoplasm Transplantation Neoplasms, Experimental - enzymology Neoplasms, Experimental - genetics Neoplasms, Experimental - pathology Nervous system Optic nerve Patients Pediatrics Real-Time Polymerase Chain Reaction Retina Retinal Neoplasms - enzymology Retinal Neoplasms - genetics Retinal Neoplasms - pathology Retinoblastoma Retinoblastoma - enzymology Retinoblastoma - genetics Retinoblastoma - secondary RNA, Messenger - genetics RNA, Messenger - metabolism Signal Transduction Trans-Activators - genetics Trans-Activators - metabolism Tumor Cells, Cultured Tumor model Tumors Xenograft
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Beschreibung
Zusammenfassung:	Highlights • Preclinical retinoblastoma models derived from heavily pretreated patients are scarce. • Novel patient-derived preclinical retinoblastoma models (cell cultures in serum-free medium and orthotopic xenografts) reproduce key histologic and genetic features of paired patient biopsies. • Molecular method (RT-qPCR detection of CRX ) quantifies local and metastatic tumor burden with high sensitivity and specificity in the new xenografts. • Our research expands the previously available preclinical tools for translational research in retinoblastoma.
ISSN:	0304-3835 1872-7980
DOI:	10.1016/j.canlet.2016.06.012