Application of flow cytometry for myelodysplastic syndromes: Pitfalls and technical considerations

The application of flow cytometry (FC) is recommended as part of the diagnostic approach for MDS. The complexity of flow cytometric analysis of bone marrow cells in MDS has been an obstacle for general application. However, in the past years several studies showed practical flow cytometric approache...

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Veröffentlicht in:	Cytometry. Part B, Clinical cytometry Clinical cytometry, 2016-07, Vol.90 (4), p.358-367
Hauptverfasser:	Alhan, Canan, Westers, Theresia M., Cremers, Eline M. P., Cali, Claudia, Ossenkoppele, Gert J., van de Loosdrecht, Arjan A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bone Marrow - metabolism Bone Marrow Cells - cytology flow cytometry Flow Cytometry - methods Humans Immunophenotyping - methods myelodysplastic syndromes Myelodysplastic Syndromes - diagnosis Myelodysplastic Syndromes - pathology pitfalls Prognosis
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Beschreibung
Zusammenfassung:	The application of flow cytometry (FC) is recommended as part of the diagnostic approach for MDS. The complexity of flow cytometric analysis of bone marrow cells in MDS has been an obstacle for general application. However, in the past years several studies showed practical flow cytometric approaches for the diagnosis and prognosis of MDS. In this report we discuss technical considerations and highlight issues that require special attention when handling and analyzing bone marrow samples of patients with cytopenia and suspicion of MDS. © 2015 Clinical Cytometry Society
ISSN:	1552-4949 1552-4957
DOI:	10.1002/cyto.b.21333