Biofilm formation in various conditions is not a key factor of persistence potential of Listeria monocytogenes in food-processing environment

In this study, a hypothesis was tested that biofilm formation ability, or ability to form biofilm in specific conditions, is responsible for persistence of Listeria monocytogenes in food processing environment. Thirty-three L. monocytogenes strains isolated from food-processing environment and food...

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Veröffentlicht in:Journal of food and nutrition research 2016-01, Vol.55 (2), p.189-193
Hauptverfasser: Korenova, J, Oravcova, K, Veghova, A, Karpiskova, R, Kuchta, T
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Sprache:eng
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Zusammenfassung:In this study, a hypothesis was tested that biofilm formation ability, or ability to form biofilm in specific conditions, is responsible for persistence of Listeria monocytogenes in food processing environment. Thirty-three L. monocytogenes strains isolated from food-processing environment and food products were tested for biofilm-forming ability in various conditions. Biofilm-forming ability was tested with exponential-phase cultures at 20 [degrees]C, 37 [degrees]C and 40 [degrees]C, with stationary-phase cultures at 20 [degrees]C, 37 [degrees]C and 40 [degrees]C, with cultures grown in limited-nutrient conditions and with cultures grown in high-salt conditions at 4 [degrees]C. Biofilm was formed in 96-well polystyrene plates and quantified by the method based on staining with crystal violet. The results showed that biofilm-forming ability of L. monocytogenes strains was variable, individual strains exhibiting different relative biofilm-forming ability in various conditions. No statistically significant differences between the groups of persistent and sporadic strains in biofilm-forming ability in any of the conditions were determined. Based on our results, biofilm-forming ability of L. monocytogenes is not a key factor that determines its persistence potential in food-processing environment.
ISSN:1336-8672