Mitochondrial oxygen consumption is a unique indicator of stallion spermatozoal health and varies with cryopreservation media

Mitochondrial oxygen consumption is a unique indicator of stallion spermatozoal health and varies with cryopreservation media

Mitochondrial oxygen consumption is a sensitive indicator of spermatozoal health in the context of cryopreservation. We investigated oxygen consumption of equine sperm mitochondria during incubation in four commercially available sperm cryopreservation extenders: modified INRA 96, BotuCrio, EZ Freez...

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Veröffentlicht in:Theriogenology 2016-09, Vol.86 (5), p.1382-1392
Hauptverfasser: Darr, Christa R., Cortopassi, Gino A., Datta, Sandipan, Varner, Dickson D., Meyers, Stuart A.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cryopreservation - veterinary
Cryopreservation extender
Cryoprotective Agents - pharmacology
Horses - physiology
Male
Mitochondria
Mitochondria - physiology
Oxygen Consumption - physiology
Semen Analysis - veterinary
Sperm
Spermatozoa - physiology
Stallion
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Zusammenfassung:Mitochondrial oxygen consumption is a sensitive indicator of spermatozoal health in the context of cryopreservation. We investigated oxygen consumption of equine sperm mitochondria during incubation in four commercially available sperm cryopreservation extenders: modified INRA 96, BotuCrio, EZ Freezin–“LE” and “MFR5”, in addition to several other parameters including motility, reactive oxygen species (ROS) production and viability. All experimental endpoints, with the exception of average path velocity, were affected significantly by freezing extender type after freezing and thawing. Sperm in INRA 96 had the lowest average progressive motility after thawing (24 ± 4.8%, P 
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2016.04.082