Estradiol-tethered micropatterned surfaces for the study of estrogenic non-genomic pathways

Besides its well-known hormonal effects initiated in the nucleus, estradiol (E2) also activates non-nuclear pathways through interactions with receptors located on the cell plasma membrane. Micropatterned substrates consisting of gold dots bearing tethered E2 distributed on a cell-adhesive substrate were prepared and shown to trigger specifically E2 non-genomic effects in cells grown on the substrates. Besides its hormonal effects, estradiol (E2) also activates non-nuclear pathways through interactions with receptors on the cell membrane.