Purification and Characterization of a Rabbit Serum Factor That Kills Listeria Species and Other Foodborne Bacterial Pathogens

In an in-vitro assay, rabbit serum, but not human serum, killed Listeria monocytogenes, a foodborne pathogen. The aim of our study was to purify and partially characterize this killing factor. Listericidin was purified from rabbit serum by a single-step ion-exchange chromatography with DEAE-Sephadex...

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Veröffentlicht in:	Foodborne pathogens and disease 2016-08, Vol.13 (8), p.441-447
Hauptverfasser:	Kothary, Mahendra H, Franco, Augusto A, Tall, Ben D, Gopinath, Gopal R, Datta, Atin R
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Anti-Bacterial Agents - blood Anti-Bacterial Agents - pharmacology Antibodies, Monoclonal - pharmacology Blood Proteins - chemistry Blood Proteins - isolation & purification Blood Proteins - pharmacology Complement C3a - chemistry Complement C3a - immunology Food Microbiology Foodborne Diseases - microbiology Hot Temperature Humans Hydrogen-Ion Concentration Listeria monocytogenes - drug effects Rabbits - blood Sequence Analysis, Protein Sequence Homology Sodium Chloride - pharmacology
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Zusammenfassung:	In an in-vitro assay, rabbit serum, but not human serum, killed Listeria monocytogenes, a foodborne pathogen. The aim of our study was to purify and partially characterize this killing factor. Listericidin was purified from rabbit serum by a single-step ion-exchange chromatography with DEAE-Sephadex A-50 and its antimicrobial activity was assessed by a microdilution method. Listericidin is a protein with a molecular weight of 9 kDa and an isoelectric point of 8.1. It kills L. monocytogenes at 4°C, 25°C, and 37°C, and its activity is resistant to heat (boiling) and acidic conditions (pH
ISSN:	1535-3141 1556-7125
DOI:	10.1089/fpd.2015.2109