The bet Gene of Feline Foamy Virus Is Required for Virus Replication

The bet Gene of Feline Foamy Virus Is Required for Virus Replication

Foamy viruses (FV) are complex retroviruses with additional bel genes located between env and the 3′ long-terminal repeat. The functions of the bel 2 and bet genes are unknown and both are dispensable for replication of the prototypic human foamy virus in cell cultures. We examined the function(s) o...

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Veröffentlicht in:Virology (New York, N.Y.) N.Y.), 2001-09, Vol.287 (2), p.310-320
Hauptverfasser: Alke, Alexandra, Schwantes, Astrid, Kido, Kenji, Flötenmeyer, Matthias, Flügel, Rolf M., Löchelt, Martin
Format: Artikel
Sprache:eng
Schlagworte:
accessory bel 2 and bet genes
Amino Acid Sequence
Animals
bel 2 gene
Bel protein
bel2 gene
bet gene
Bet protein
Cat Diseases - virology
Cats
Cells, Cultured
env gene
Feline foamy virus
Gene Deletion
Gene Expression
Humans
Molecular Sequence Data
Mutation
Proviruses - genetics
Proviruses - ultrastructure
spumaretrovirus
Spumavirus - genetics
Spumavirus - physiology
Spumavirus - ultrastructure
Terminal Repeat Sequences
Viral Proteins - genetics
Virion - physiology
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Zusammenfassung:Foamy viruses (FV) are complex retroviruses with additional bel genes located between env and the 3′ long-terminal repeat. The functions of the bel 2 and bet genes are unknown and both are dispensable for replication of the prototypic human foamy virus in cell cultures. We examined the function(s) of bel 2 and bet of the distantly related feline foamy virus (FFV) in the proviral context. Mutagenesis was used to alter the Bel 2 and Bet or to abrogate their expression. The Bel 2/Bet mutants showed a 1000-fold reduced viral titer in feline kidney cells; in human 293T cells, viral titer was only about 10-fold reduced compared to wild-type FFV. In both cell types, the Bel 2/Bet mutations resulted in a reduced release of FFV particles. The results indicate that FFV Bet is required for efficient virus replication. The functions of the Bel 2 and Bet proteins are discussed.
ISSN:0042-6822
1096-0341
DOI:10.1006/viro.2001.1065