Evaluation of the Red Blood Cell Advanced Software Application on the CellaVision DM96
Summary Introduction The CellaVision Advanced Red Blood Cell (RBC) Software Application is a new software for advanced morphological analysis of RBCs on a digital microscopy system. Upon automated precharacterization into 21 categories, the software offers the possibility of reclassification of RBCs...
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Veröffentlicht in: | International journal of laboratory hematology 2016-08, Vol.38 (4), p.366-374 |
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Sprache: | eng |
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Zusammenfassung: | Summary
Introduction
The CellaVision Advanced Red Blood Cell (RBC) Software Application is a new software for advanced morphological analysis of RBCs on a digital microscopy system. Upon automated precharacterization into 21 categories, the software offers the possibility of reclassification of RBCs by the operator. We aimed to define the optimal cut‐off to detect morphological RBC abnormalities and to evaluate the precharacterization performance of this software.
Methods
Thirty‐eight blood samples of healthy donors and sixty‐eight samples of hospitalized patients were analyzed. Different methodologies to define a cut‐off between negativity and positivity were used. Sensitivity and specificity were calculated according to these different cut‐offs using the manual microscopic method as the gold standard. Imprecision was assessed by measuring analytical within‐run and between‐run variability and by measuring between‐observer variability.
Results
By optimizing the cut‐off between negativity and positivity, sensitivities exceeded 80% for ‘critical’ RBC categories (target cells, tear drop cells, spherocytes, sickle cells, and parasites), while specificities exceeded 80% for the other RBC morphological categories. Results of within‐run, between‐run, and between‐observer variabilities were all clinically acceptable.
Conclusion
The CellaVision Advanced RBC Software Application is an easy‐to‐use software that helps to detect most RBC morphological abnormalities in a sensitive and specific way without increasing work load, provided the proper cut‐offs are chosen. However, evaluation of the images by an experienced observer remains necessary. |
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ISSN: | 1751-5521 1751-553X |
DOI: | 10.1111/ijlh.12497 |