Prostaglandin E2 elevates calcium in differentiated neuroectodermal stem cells
Lipid mediator prostaglandin E2 (PGE2) is an endogenous signaling molecule that plays an important role during early development of the nervous system. Abnormalities in the PGE2 signaling pathway have been associated with neurodevelopmental disorders such as autism spectrum disorders. In this study...
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Veröffentlicht in: | Molecular and cellular neuroscience 2016-07, Vol.74, p.71-77 |
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Sprache: | eng |
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Zusammenfassung: | Lipid mediator prostaglandin E2 (PGE2) is an endogenous signaling molecule that plays an important role during early development of the nervous system. Abnormalities in the PGE2 signaling pathway have been associated with neurodevelopmental disorders such as autism spectrum disorders. In this study we use ratiometric fura-2AM calcium imaging to show that higher levels of PGE2 elevate intracellular calcium levels in the cell soma and growth cones of differentiated neuroectodermal (NE-4C) stem cells. PGE2 also increased the amplitude of calcium fluctuation in the neuronal growth cones and affected the neurite extension length. In summary, our results show that PGE2 may adversely impact intracellular calcium dynamics in differentiated neuronal cells and possibly affect early development of the nervous system.
•This study conducted in differentiated neuroectodermal stem cells shows that the lipid mediator prostaglandin E2 alters:•intracellular calcium levels in the cytosol and growth cones•the calcium fluctuation transients in the growth cone, and•the length of neuronal extensions•these findings can have implications during early brain development in gene expression, synaptic activity, and axon guidance |
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ISSN: | 1044-7431 1095-9327 |
DOI: | 10.1016/j.mcn.2016.03.010 |