Role of IL-6 in Progress of Estrogen Promoting Lung Adenocarcinoma in Mice and Its Mechanism

Role of IL-6 in Progress of Estrogen Promoting Lung Adenocarcinoma in Mice and Its Mechanism

Objective To explore the role of IL-6 in the progress of Estrogen(E2) promoting lung adenocarcinoma in mice and its possible mechanism. Methods Urethane-induced lung adenocarcinoma models of female Kunming mice were established, and divided as follows(n=9 in each group): the control, E2, E2+estrogen...

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Veröffentlicht in:Zhongliu fangzhi yanjiu 2016-01, Vol.43 (4)
Hauptverfasser: Ren, Nili, Chen, Ping
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description Objective To explore the role of IL-6 in the progress of Estrogen(E2) promoting lung adenocarcinoma in mice and its possible mechanism. Methods Urethane-induced lung adenocarcinoma models of female Kunming mice were established, and divided as follows(n=9 in each group): the control, E2, E2+estrogen inhibitors(E2I), E2I, IL-6 and E2+IL-6 group. 16 weeks later, we sacrificed mice and tested weight changes, tumorigenicity, the total number of nodules, tumor grade, and lung indices. At the same time, we detected the content of E2/IL-6 in serum specimens by enzyme-linked immunoassay (ELISA), ER[beta]/IL-6 mRNA levels by RT-PCR, and the protein expression of ER[beta], Akt, MAPK, p-ER beta, p-Akt, p-MAPK by Western blot. Results The pulmonary nodules rate in Kunming mice were 87.04% (47/54); the lung adenocarcinoma rate were 70.37%(38/54); the total number of nodules, tumor grade, and lung indices in E2 group were significantly higher than those in E2+E2I and E2I group (P
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Methods Urethane-induced lung adenocarcinoma models of female Kunming mice were established, and divided as follows(n=9 in each group): the control, E2, E2+estrogen inhibitors(E2I), E2I, IL-6 and E2+IL-6 group. 16 weeks later, we sacrificed mice and tested weight changes, tumorigenicity, the total number of nodules, tumor grade, and lung indices. At the same time, we detected the content of E2/IL-6 in serum specimens by enzyme-linked immunoassay (ELISA), ER[beta]/IL-6 mRNA levels by RT-PCR, and the protein expression of ER[beta], Akt, MAPK, p-ER beta, p-Akt, p-MAPK by Western blot. Results The pulmonary nodules rate in Kunming mice were 87.04% (47/54); the lung adenocarcinoma rate were 70.37%(38/54); the total number of nodules, tumor grade, and lung indices in E2 group were significantly higher than those in E2+E2I and E2I group (P&lt;0.05); and above detection index in E2 grou</description><identifier>ISSN: 1000-8578</identifier><language>chi</language><ispartof>Zhongliu fangzhi yanjiu, 2016-01, Vol.43 (4)</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids></links><search><creatorcontrib>Ren, Nili</creatorcontrib><creatorcontrib>Chen, Ping</creatorcontrib><title>Role of IL-6 in Progress of Estrogen Promoting Lung Adenocarcinoma in Mice and Its Mechanism</title><title>Zhongliu fangzhi yanjiu</title><description>Objective To explore the role of IL-6 in the progress of Estrogen(E2) promoting lung adenocarcinoma in mice and its possible mechanism. 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Results The pulmonary nodules rate in Kunming mice were 87.04% (47/54); the lung adenocarcinoma rate were 70.37%(38/54); the total number of nodules, tumor grade, and lung indices in E2 group were significantly higher than those in E2+E2I and E2I group (P&lt;0.05); and above detection index in E2 grou</description><issn>1000-8578</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNqVi80KgkAUhWdRkJTvMMs2wjXzZxthJChEtAxkGq82MM6Ud3z_NHqBNufjfJyzYF4IAEEWp9mK-UTqARCGsI9S8Nj9ajVy2_KiDBKuDL8MthuQaHY5uanhV_bWKdPxcpzi0KCxUgxSGduL-VUpiVyYhheOeIXyKYyifsOWrdCE_o9rtj3lt-M5eA32PSK5ulckUWth0I5UhxlkSRpDuov-mH4Av15GFQ</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>Ren, Nili</creator><creator>Chen, Ping</creator><scope>7TO</scope><scope>H94</scope></search><sort><creationdate>20160101</creationdate><title>Role of IL-6 in Progress of Estrogen Promoting Lung Adenocarcinoma in Mice and Its Mechanism</title><author>Ren, Nili ; Chen, Ping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_miscellaneous_18086750723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2016</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ren, Nili</creatorcontrib><creatorcontrib>Chen, Ping</creatorcontrib><collection>Oncogenes and Growth Factors Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Zhongliu fangzhi yanjiu</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ren, Nili</au><au>Chen, Ping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of IL-6 in Progress of Estrogen Promoting Lung Adenocarcinoma in Mice and Its Mechanism</atitle><jtitle>Zhongliu fangzhi yanjiu</jtitle><date>2016-01-01</date><risdate>2016</risdate><volume>43</volume><issue>4</issue><issn>1000-8578</issn><abstract>Objective To explore the role of IL-6 in the progress of Estrogen(E2) promoting lung adenocarcinoma in mice and its possible mechanism. 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title Role of IL-6 in Progress of Estrogen Promoting Lung Adenocarcinoma in Mice and Its Mechanism
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