Role of IL-6 in Progress of Estrogen Promoting Lung Adenocarcinoma in Mice and Its Mechanism

Objective To explore the role of IL-6 in the progress of Estrogen(E2) promoting lung adenocarcinoma in mice and its possible mechanism. Methods Urethane-induced lung adenocarcinoma models of female Kunming mice were established, and divided as follows(n=9 in each group): the control, E2, E2+estrogen inhibitors(E2I), E2I, IL-6 and E2+IL-6 group. 16 weeks later, we sacrificed mice and tested weight changes, tumorigenicity, the total number of nodules, tumor grade, and lung indices. At the same time, we detected the content of E2/IL-6 in serum specimens by enzyme-linked immunoassay (ELISA), ER[beta]/IL-6 mRNA levels by RT-PCR, and the protein expression of ER[beta], Akt, MAPK, p-ER beta, p-Akt, p-MAPK by Western blot. Results The pulmonary nodules rate in Kunming mice were 87.04% (47/54); the lung adenocarcinoma rate were 70.37%(38/54); the total number of nodules, tumor grade, and lung indices in E2 group were significantly higher than those in E2+E2I and E2I group (P