Silencing of CtBP1 suppresses the migration in human glioma cells
Carboxyl-terminal binding protein 1 (CtBP1), up-regulated in various types of human cancers, has been functionally associated with proliferation, anti-apoptosis, and EMT in vitro studies. However, the functional significance of CtBP1 in the pathophysiology of glioma remains unknown. In the present s...
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| Veröffentlicht in: | Journal of molecular histology 2016-06, Vol.47 (3), p.297-304 |
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| creator | Zhao, Chengjin Shen, Yifen Tao, Xuelei Xu, Jian Lu, Junjie Liu, Chao Xu, Zhiwei Tang, Qing Tao, Tao Zhang, Xiubing |
| description | Carboxyl-terminal binding protein 1 (CtBP1), up-regulated in various types of human cancers, has been functionally associated with proliferation, anti-apoptosis, and EMT in vitro studies. However, the functional significance of CtBP1 in the pathophysiology of glioma remains unknown. In the present study, we showed the expression of CtBP1 was markedly higher in glioma tissues compared with normal brain tissues by Western blot analysis. Immunohistochemical analysis revealed that CtBP1 mainly localized in the nucleus of glioma cells. Statistical analysis suggested the upregulation of CtBP1 was considerably correlated with the WHO grade (
P
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| doi_str_mv | 10.1007/s10735-016-9678-z |
| format | Article |
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P
< 0.05) and those patients with high CtBP1 levels exhibited shorter survival time (
P
< 0.01). Silencing CtBP1 by short hairpin RNAi caused an inhibition of cell migration. Moreover, knockdown of CtBP1 increases E-cadherin expression and decreases vimentin expression. These data uncovered that CtBP1 protein is a valuable marker of glioma pathogenic process and that CtBP1 can serve as a novel prognostic marker for glioma therapy.</description><identifier>ISSN: 1567-2379</identifier><identifier>EISSN: 1567-2387</identifier><identifier>DOI: 10.1007/s10735-016-9678-z</identifier><identifier>PMID: 27160109</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Adult ; Aged ; Alcohol Oxidoreductases - genetics ; Apoptosis ; Biomedical and Life Sciences ; Biomedicine ; Cell Biology ; Cell Line, Tumor ; Cell Movement - genetics ; Cell Proliferation ; Developmental Biology ; DNA-Binding Proteins - genetics ; Female ; Gene Expression ; Gene Silencing ; Glioma - genetics ; Glioma - mortality ; Glioma - pathology ; Glioma - therapy ; Humans ; Kaplan-Meier Estimate ; Life Sciences ; Male ; Middle Aged ; Neoplasm Grading ; Neoplasm Staging ; Original Paper ; Prognosis</subject><ispartof>Journal of molecular histology, 2016-06, Vol.47 (3), p.297-304</ispartof><rights>Springer Science+Business Media Dordrecht 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-49223e336fa2647281880acad130e1e003521b27e99e839382c5d2f533a0a7d53</citedby><cites>FETCH-LOGICAL-c405t-49223e336fa2647281880acad130e1e003521b27e99e839382c5d2f533a0a7d53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10735-016-9678-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10735-016-9678-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27160109$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Chengjin</creatorcontrib><creatorcontrib>Shen, Yifen</creatorcontrib><creatorcontrib>Tao, Xuelei</creatorcontrib><creatorcontrib>Xu, Jian</creatorcontrib><creatorcontrib>Lu, Junjie</creatorcontrib><creatorcontrib>Liu, Chao</creatorcontrib><creatorcontrib>Xu, Zhiwei</creatorcontrib><creatorcontrib>Tang, Qing</creatorcontrib><creatorcontrib>Tao, Tao</creatorcontrib><creatorcontrib>Zhang, Xiubing</creatorcontrib><title>Silencing of CtBP1 suppresses the migration in human glioma cells</title><title>Journal of molecular histology</title><addtitle>J Mol Hist</addtitle><addtitle>J Mol Histol</addtitle><description>Carboxyl-terminal binding protein 1 (CtBP1), up-regulated in various types of human cancers, has been functionally associated with proliferation, anti-apoptosis, and EMT in vitro studies. However, the functional significance of CtBP1 in the pathophysiology of glioma remains unknown. In the present study, we showed the expression of CtBP1 was markedly higher in glioma tissues compared with normal brain tissues by Western blot analysis. Immunohistochemical analysis revealed that CtBP1 mainly localized in the nucleus of glioma cells. Statistical analysis suggested the upregulation of CtBP1 was considerably correlated with the WHO grade (
P
< 0.05) and those patients with high CtBP1 levels exhibited shorter survival time (
P
< 0.01). Silencing CtBP1 by short hairpin RNAi caused an inhibition of cell migration. Moreover, knockdown of CtBP1 increases E-cadherin expression and decreases vimentin expression. These data uncovered that CtBP1 protein is a valuable marker of glioma pathogenic process and that CtBP1 can serve as a novel prognostic marker for glioma therapy.</description><subject>Adult</subject><subject>Aged</subject><subject>Alcohol Oxidoreductases - genetics</subject><subject>Apoptosis</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Biology</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement - genetics</subject><subject>Cell Proliferation</subject><subject>Developmental Biology</subject><subject>DNA-Binding Proteins - genetics</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Gene Silencing</subject><subject>Glioma - genetics</subject><subject>Glioma - mortality</subject><subject>Glioma - pathology</subject><subject>Glioma - therapy</subject><subject>Humans</subject><subject>Kaplan-Meier Estimate</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Neoplasm Grading</subject><subject>Neoplasm Staging</subject><subject>Original Paper</subject><subject>Prognosis</subject><issn>1567-2379</issn><issn>1567-2387</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkUtLxTAQhYMoXl8_wI0E3LipziRtHsvrxRcICuo6xDa9Vvq4Ju1Cf70pvYoI4ioD-ebMzDmEHCKcIoA8CwiSZwmgSLSQKvnYIDuYCZkwruTmdy31jOyG8ArAlEj1NpkxiQIQ9A6ZP1S1a_OqXdKupIv-_B5pGFYr70JwgfYvjjbV0tu-6lpatfRlaGxLl3XVNZbmrq7DPtkqbR3cwfrdI0-XF4-L6-T27upmMb9N8hSyPkk1Y9xxLkrLRCqZQqXA5rZADg4dAM8YPjPptHaKa65YnhWszDi3YGWR8T1yMumufPc2uNCbpgrjBrZ13RAMKlBCokb4H5U6ugJxiYge_0Jfu8G38ZCRigYKnY0UTlTuuxC8K83KV4317wbBjFGYKQoTozBjFOYj9hytlYfnxhXfHV_eR4BNQIhf7dL5H6P_VP0EwnORGA</recordid><startdate>20160601</startdate><enddate>20160601</enddate><creator>Zhao, Chengjin</creator><creator>Shen, Yifen</creator><creator>Tao, Xuelei</creator><creator>Xu, Jian</creator><creator>Lu, Junjie</creator><creator>Liu, Chao</creator><creator>Xu, Zhiwei</creator><creator>Tang, Qing</creator><creator>Tao, Tao</creator><creator>Zhang, Xiubing</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20160601</creationdate><title>Silencing of CtBP1 suppresses the migration in human glioma cells</title><author>Zhao, Chengjin ; Shen, Yifen ; Tao, Xuelei ; Xu, Jian ; Lu, Junjie ; Liu, Chao ; Xu, Zhiwei ; Tang, Qing ; Tao, Tao ; Zhang, Xiubing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-49223e336fa2647281880acad130e1e003521b27e99e839382c5d2f533a0a7d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Alcohol Oxidoreductases - genetics</topic><topic>Apoptosis</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell Biology</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement - genetics</topic><topic>Cell Proliferation</topic><topic>Developmental Biology</topic><topic>DNA-Binding Proteins - genetics</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Gene Silencing</topic><topic>Glioma - genetics</topic><topic>Glioma - mortality</topic><topic>Glioma - pathology</topic><topic>Glioma - therapy</topic><topic>Humans</topic><topic>Kaplan-Meier Estimate</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Neoplasm Grading</topic><topic>Neoplasm Staging</topic><topic>Original Paper</topic><topic>Prognosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Chengjin</creatorcontrib><creatorcontrib>Shen, Yifen</creatorcontrib><creatorcontrib>Tao, Xuelei</creatorcontrib><creatorcontrib>Xu, Jian</creatorcontrib><creatorcontrib>Lu, Junjie</creatorcontrib><creatorcontrib>Liu, Chao</creatorcontrib><creatorcontrib>Xu, Zhiwei</creatorcontrib><creatorcontrib>Tang, Qing</creatorcontrib><creatorcontrib>Tao, Tao</creatorcontrib><creatorcontrib>Zhang, Xiubing</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular histology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Chengjin</au><au>Shen, Yifen</au><au>Tao, Xuelei</au><au>Xu, Jian</au><au>Lu, Junjie</au><au>Liu, Chao</au><au>Xu, Zhiwei</au><au>Tang, Qing</au><au>Tao, Tao</au><au>Zhang, Xiubing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Silencing of CtBP1 suppresses the migration in human glioma cells</atitle><jtitle>Journal of molecular histology</jtitle><stitle>J Mol Hist</stitle><addtitle>J Mol Histol</addtitle><date>2016-06-01</date><risdate>2016</risdate><volume>47</volume><issue>3</issue><spage>297</spage><epage>304</epage><pages>297-304</pages><issn>1567-2379</issn><eissn>1567-2387</eissn><abstract>Carboxyl-terminal binding protein 1 (CtBP1), up-regulated in various types of human cancers, has been functionally associated with proliferation, anti-apoptosis, and EMT in vitro studies. However, the functional significance of CtBP1 in the pathophysiology of glioma remains unknown. In the present study, we showed the expression of CtBP1 was markedly higher in glioma tissues compared with normal brain tissues by Western blot analysis. Immunohistochemical analysis revealed that CtBP1 mainly localized in the nucleus of glioma cells. Statistical analysis suggested the upregulation of CtBP1 was considerably correlated with the WHO grade (
P
< 0.05) and those patients with high CtBP1 levels exhibited shorter survival time (
P
< 0.01). Silencing CtBP1 by short hairpin RNAi caused an inhibition of cell migration. Moreover, knockdown of CtBP1 increases E-cadherin expression and decreases vimentin expression. These data uncovered that CtBP1 protein is a valuable marker of glioma pathogenic process and that CtBP1 can serve as a novel prognostic marker for glioma therapy.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>27160109</pmid><doi>10.1007/s10735-016-9678-z</doi><tpages>8</tpages></addata></record> |
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| subjects | Adult Aged Alcohol Oxidoreductases - genetics Apoptosis Biomedical and Life Sciences Biomedicine Cell Biology Cell Line, Tumor Cell Movement - genetics Cell Proliferation Developmental Biology DNA-Binding Proteins - genetics Female Gene Expression Gene Silencing Glioma - genetics Glioma - mortality Glioma - pathology Glioma - therapy Humans Kaplan-Meier Estimate Life Sciences Male Middle Aged Neoplasm Grading Neoplasm Staging Original Paper Prognosis |
| title | Silencing of CtBP1 suppresses the migration in human glioma cells |
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