d-Allulose supplementation normalized the body weight and fat-pad mass in diet-induced obese mice via the regulation of lipid metabolism under isocaloric fed condition

Scope A number of findings suggest that zero‐calorie d‐allulose, also known as d‐psicose, has beneficial effects on obesity‐related metabolic disturbances. However, it is unclear whether d‐allulose can normalize the metabolic status of diet‐induced obesity without having an impact on the energy density. We investigated whether 5% d‐allulose supplementation in a high fat diet(HFD) could normalize body fat in a diet‐induced obesity animal model under isocaloric pair‐fed conditions. Methods and results Mice were fed an HFD with or without various sugar substitutes (d‐glucose, d‐fructose, erytritol, or d‐allulose, n = 10 per group) for 16 wk. Body weight and fat‐pad mass in the d‐allulose group were dramatically lowered to that of the normal group with a simultaneous decrease in plasma leptin and resistin concentrations. d‐allulose lowered plasma and hepatic lipids while elevating fecal lipids with a decrease in mRNA expression of CD36, ApoB48, FATP4, in the small intestine in mice. In the liver, activities of both fatty acid synthase and β‐oxidation were downregulated by d‐allulose to that of the normal group; however, in WAT, fatty acid synthase was decreased while β‐oxidation activity was enhanced. Conclusion Taken together, our findings suggest that 5% dietary d‐allulose led to the normalization of the metabolic status of diet‐induced obesity by altering lipid‐regulating enzyme activities and their gene‐expression level along with fecal lipids. We investigated whether 5% d‐allulose supplementation in a high fat diet could normalize body fat in a diet‐induced obesity animal model under isocaloric pair‐fed conditions. d‐allulose lowered plasma and hepatic lipids while elevating fecal lipids in the small intestine in mice. In the liver, activities of both fatty acid synthase and β‐oxidation were downregulated by d‐allulose to that of the normal group; however, in white adipose tissue, fatty acid synthase was decreased while β‐oxidation activity was enhanced.