Comprehensive kinetic and structural studies of different flavonoids inhibiting diphenolase activity of mushroom tyrosinase
The effect of 4 flavonoids on the diphenolase activity of mushroom tyrosinase was studied using spectroscopic approach. Analysis of kinetic data demonstrated that flavonoids cause a reversible inhibition of the enzyme activity. Further study showed that gallic acid acted as noncompetitive inhibitor,...
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Veröffentlicht in: | Applied biochemistry and microbiology 2016-05, Vol.52 (3), p.304-310 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The effect of 4 flavonoids on the diphenolase activity of mushroom tyrosinase was studied using spectroscopic approach. Analysis of kinetic data demonstrated that flavonoids cause a reversible inhibition of the enzyme activity. Further study showed that gallic acid acted as noncompetitive inhibitor, whereas chrysin, naringin and quercetin inhibited the diphenolase activity of mushroom tyrosinase in a competitive fashion. Comparison of the inhibition constants revealed that the strength with which the inhibitors acted on the enzyme activity was ranking as follows: chrysin (
K
i
7.90 mM) < quercetin (
K
i
7.44 mM) < naringin (
K
i
3.04 mM) < gallic acid (
K
i
1.5 mM). These data, therefore, suggest that gallic acid is the most potent inhibitor of the enzyme compared to the other flavonoids used. |
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ISSN: | 0003-6838 1608-3024 |
DOI: | 10.1134/S0003683816030054 |