Whole genome sequence of Pantoea ananatis R100, an antagonistic bacterium isolated from rice seed

•We present complete genome of Pantoea ananatis R100, which is an antagonistic bacterium.•Many secondary metabolism gene clusters, including oxazolomycin and chalcomycin biosynthesis gene clusters, which contributed to the biocontrol ability, were found in R100.•Only one type III secretion system coding gene is exist in R100, indicating its non-pathogenicity on plant and human. Pantoea ananatis is a group of bacteria, which was first reported as plant pathogen. Recently, several papers also described its biocontrol ability. In 2003, P. ananatis R100, which showed strong antagonism against several plant pathogens, was isolated from rice seeds. In this study, whole genome sequence of this strain was determined by SMRT Cell technology. The total genome size of R100 is 4,857,861bp with 4659 coding genes (CDS), 82 tRNAs and 22 rRNAs. The genome sequence of R100 may shed a light on the research of antagonism P. ananatis.