A Rapid and Automated Device for Purifying Nucleic Acids

We have developed a rapid, automated nucleic acid purification device in a single cartridge containing silica-coated magnetic beads. We succeeded in extracting the matrix protein gene of influenza A virus from pharyngeal swab samples within 3 min. The device will be widely applicable to detect a spe...

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Veröffentlicht in:	Analytical Sciences 2016/03/10, Vol.32(3), pp.371-374
Hauptverfasser:	UEHARA, Masayuki, HANAMURA, Masato, YAMADA, Kiyohito, YAMAGUCHI, Akemi, MURAYAMA, Toshiro, SAITO, Yuji, IDEGAMI, Kotaro, HONDA, Takayuki
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical Chemistry Automation Automation, Laboratory - instrumentation cartridge Cartridges Chemistry Devices Equipment Design extraction Genes Genetics Humans Influenza Influenza A virus Influenza A virus - genetics Influenza, Human - virology magnetic beads Magnetics Nucleic acid Nucleic acids Nucleic Acids - isolation & purification Pharynx - virology polymerase chain reaction Reverse Transcriptase Polymerase Chain Reaction RNA, Viral - isolation & purification Sensitivity and Specificity Silica Time Factors Viruses
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Beschreibung
Zusammenfassung:	We have developed a rapid, automated nucleic acid purification device in a single cartridge containing silica-coated magnetic beads. We succeeded in extracting the matrix protein gene of influenza A virus from pharyngeal swab samples within 3 min. The device will be widely applicable to detect a specific gene from the various samples for clinical diagnosis and genetic research.
ISSN:	0910-6340 1348-2246
DOI:	10.2116/analsci.32.371