Conversion of a non-heme iron-dependent sulfoxide synthase into a thiol dioxygenase by a single point mutation

Conversion of a non-heme iron-dependent sulfoxide synthase into a thiol dioxygenase by a single point mutation

EgtB from Mycobacterium thermoresistibile catalyzes O2-dependent sulfur-carbon bond formation between the side chains of Nα-trimethyl histidine and γ-glutamyl cysteine as a central step in ergothioneine biosynthesis. A single point mutation converts this enzyme into a γ-glutamyl cysteine dioxygenase...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Chemical communications (Cambridge, England) England), 2016-01, Vol.52 (9), p.1945-1948
Hauptverfasser: Goncharenko, Kristina V, Seebeck, Florian P
Format: Artikel
Sprache:eng
Schlagworte:
Bonding
Catalytic Domain
Conversion
Cysteine
Dioxygenases - chemistry
Dioxygenases - genetics
Enzymes
Evolution
Histidine
Iron - chemistry
Mutations
Point Mutation
Thiols
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:EgtB from Mycobacterium thermoresistibile catalyzes O2-dependent sulfur-carbon bond formation between the side chains of Nα-trimethyl histidine and γ-glutamyl cysteine as a central step in ergothioneine biosynthesis. A single point mutation converts this enzyme into a γ-glutamyl cysteine dioxygenase with an efficiency that rivals naturally evolved thiol dioxygenases.
ISSN:1359-7345
1364-548X
DOI:10.1039/c5cc07772a