The biogenic synthesis of a reduced graphene oxide–silver (RGO–Ag) nanocomposite and its dual applications as an antibacterial agent and cancer biomarker sensor

Cancer nanotechnology encourages cutting edge research utilizing nanomaterials for the diagnosis, therapy and prevention of cancer. Recognition of cancer-related biomarkers in the body has made early detection possible and thus, paves the way towards devising methods to control it from progressing to advanced stages. Hydrogen peroxide (H sub(2)O sub(2)) is a critical biomolecule, which plays an important dual role in cancer progression. Herein, we have developed a sensitive method for the detection of H sub(2)O sub(2) utilizing a reduced graphene oxide-silver (RGO-Ag) nanocomposite. This RGO-Ag nanocomposite was prepared using a green and facile one-step synthesis approach utilizing the extract of a medicinal mushroom, Ganoderma lucidum. The higher content of polysaccharides in this extract makes it a potent reducing agent for the combined reduction of GO and AgNO sub(3) to produce the RGO-Ag nanocomposite. The properties of the RGO-Ag obtained were characterized by UV-Vis spectroscopy, SEM, TEM, XRD, FT-IR and XPS techniques. The RGO-Ag modified electrode showed good electrocatalytic activity towards H sub(2)O sub(2) when compared to other modified electrodes. Furthermore, it showed an LOD of 136 nM, which was determined using the LSV technique. The amperometric i-tcurve displayed two different linear ranges of 1-100 mu M and 100-1100 mu M with an LOD of 3 and 56 nM, respectively. This excellent electrochemical performance towards H sub(2)O sub(2) detection could contribute to advances in current cancer diagnosis. The RGO-Ag nanocomposite was also explored as a potential antibacterial agent. Owing to its synergistic effects, RGO-Ag showed a comparable antibacterial activity to the standard antibiotic, chloramphenicol. The combined antibacterial effects and sensing potential make this RGO-Ag nanocomposite a promising candidate for future health care.