Retrofit Device for Real-Time Evaluation of Metabolic Activity in Herpes Virus Infected Cell Cultures

A speckle interferometry device for evaluation of the metabolic activity of cells cultivated on a transparent substrate was upgraded, fine-tuned, and tested. The cell cultures L-41 CD/84, Vero, and LEH-3 infected with herpes simplex virus (HSV) and placed in a cultivation medium were studied. The up...

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Veröffentlicht in:	Biomedical engineering 2014-11, Vol.48 (4), p.178-181
Hauptverfasser:	Vladimirov, A. P., Malygin, A. S., Mikhailova, Yu. A., Bakharev, A. A., Poryvaeva, A. P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Biomaterials Biomedical engineering Biomedical Engineering and Bioengineering Biotechnology Cellular biology Cultivation Devices Engineering Herpes Herpes simplex Herpes simplex virus Herpes viruses Herpesvirus Herpesvirus diseases Metabolism Real time Retrofitting Signal processing Speckle interferometry Substrates Upgrading Viruses
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Beschreibung
Zusammenfassung:	A speckle interferometry device for evaluation of the metabolic activity of cells cultivated on a transparent substrate was upgraded, fine-tuned, and tested. The cell cultures L-41 CD/84, Vero, and LEH-3 infected with herpes simplex virus (HSV) and placed in a cultivation medium were studied. The upgrading included introduction of time averaging of the signals and real time signal processing. It was demonstrated that the degree of correlation of fragments of the speckle image taken at two moments in time could be used as a parameter characterizing the metabolic activity of the cells.
ISSN:	0006-3398 1573-8256
DOI:	10.1007/s10527-014-9447-9